(a) Histological evaluation with aldehyde fuchsin
and alcian blue to identify sGAG at day 0 and day 21; deep blue/purple staining indicates sGAG accumulation and light blue staining indicates residual alginate.
ZN staining (4): Smears were flooded with filtered 1% carbol fuchsin
(CF) and heated until they were steamed and left to steam for 5 min.
BF = Basic fuchsin
at 20l/ml (1/50,000 w/v)###Phages: Wb: Weybourne
After 24 h of feeding activity, the immature soybean pods were taken out and immersed in an acid fuchsin
solution (1g of fuchsin
+ 2 ml of alcohol + 300 mL of water) for 1 h to stain the stylet sheaths.
After heat fixation, specimens were washed with carbol fuchsin
for 4 minutes and incubated with HCL.
The slices were ground and polished up to 120 [micro]m between two sheets of ground glass, stained with 1% Methylen Blue (85662, MERCK, Hohenbrunn, Germany) and 1% Fuchsin
(B-2340, Sigma Aldrich, St-Louis, USA), and finally mounted on glass.
Semithin sections were stained with toluidine blue/acid fuchsin
to obtain resolution of intracellular structures (Figures 2(a)-2(d)).
The smear on slide was fixed with methanol for 10 min and 5-7 drops of carbol fuchsin
were flooded for 2-3 minutes.
Heine technique of negative staining uses carbol fuchsin
for staining the stool smear.
The pollen mother cells were stained with improved phenol fuchsin
. Observations of the chromosome pairing of meiosis were made and documented with an Olympus BX-51 microscope coupled with a Photometric SenSys CCD camera.
These hydrogels were used for adsorption of two important synthetic dyes, i.e., basic fuchsin
and methyl violet from water.
The original method of Masson (1928) was initially thought for carcinoids analysis, integrating four dyes (Regaud's hematoxylin, acid fuchsin
with Ponceau de xylidine and aniline blue) which have been repeatedly combined on later modifications.