freeze-fracturing

freeze-fracturing

 [frēz´-frak″chur-ing]
a method of preparing cells for electron-microscopical examination: a tissue specimen is frozen at −150°C, inserted into a vacuum chamber, and fractured by a microtome; a platinum carbon replica of the exposed surfaces is made, freed of the underlying specimen, and then examined.
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Whether these through-going holes were pre-existing flaws or artifacts of the freeze-fracturing is uncertain.
The barrier performance of latex rubber
Now, a comparatively new technique called freeze-fracturing is offering dramatic new clues as to how nerve fibers are organized and what mechanisms may be responsible for producing the neurological changes seen when the myelin sheath surrounding them is damaged.
Freeze-fracture: probing the myelin depths

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