freeze-fracturing
freeze-fracturing
[frēz´-frak″chur-ing]a method of preparing cells for electron-microscopical examination: a tissue specimen is frozen at −150°C, inserted into a vacuum chamber, and fractured by a microtome; a platinum carbon replica of the exposed surfaces is made, freed of the underlying specimen, and then examined.
Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. © 2003 by Saunders, an imprint of Elsevier, Inc. All rights reserved.