fragility test


Also found in: Encyclopedia.

fra·gil·i·ty test

a test that measures the resistance of erythrocytes to hemolysis in hypotonic saline solutions; erythrocytes to be tested are added to varying concentrations of saline (usually ranging from 0.85-0.10% sodium chloride with 0.05% increments), and beginning and complete hemolysis are measured; normal erythrocytes show initial hemolysis at concentrations of 0.45-0.39% and complete hemolysis at 0.33-0.30%; in hereditary spherocytosis, the fragility of the erythrocytes is markedly increased, whereas in thalassemia, sickle cell anemia, and obstructive jaundice the fragility of the erythrocytes is usually reduced.

fragility test

(1) Capillary fragility test, see there.  
(2) Osmotic fragility test, see there.

fra·gil·i·ty test

(fră-jil'i-tē test)
A measurement of the resistance of erythrocytes to hemolysis in hypotonic saline solutions; erythrocytes to be tested are added to varying concentrations of saline and beginning and complete hemolysis are measured; in hereditary spherocytosis, the fragility of the erythrocytes is markedly increased, whereas in thalassemia and sickle cell anemia, the fragility of the erythrocytes is usually reduced.
References in periodicals archive ?
For example, in rural area where equipment or expertise is scarce, One Tube Osmotic Fragility Test (OTOFT) is a simple test that can be done to examine carriers [23].
Naked eye single tube red cell osmotic fragility test for beta thalassemia population survey.
The primary tests include osmotic fragility test, hemoglobin electrophoresis, G6PD and PK enzyme levels.
Results of modified one tube osmotic fragility test (OF) and dichlorophenolindophenol precipitate (DCIP) test, hematological analyses for 12 participants.
A A peripheral blood osmotic fragility test has traditionally been considered important in the workup of hereditary spherocytosis (HS).
There are several different tests available for HS, including the osmotic fragility test, acidified glycerol lysis test, osmotic gradient ektacytometry, hypertonic cryohaemolysis test, and eosin-5-maleimide binding test.
If these laboratory tests are suggestive and there is a family history of anemia, you still may wish to perform a definitive osmotic fragility test. Treatment for this condition is splenectomy after which spherocytosis persists, but the anemia, if it was present, improves.
Renal function tests, haemoglobin electrophoresis, Coomb's and osmotic fragility tests done in the past were normal.
Screening for thalassemias and hemoglobinopathies was carried out by microscopic examination of a stained peripheral slide, osmotic fragility tests, measurement of serum ferritin, cation exchange high performance liquid chromatography (HPLC) for Hb variants quantitation, alkaline and acid pH electrophoresis, sickle test, and DNA analysis when required.