The fractions were collected at 15 min intervals with a fraction collector
(Amersham Pharmacia Biotech, Uppsala, Sweden) and all fractions were subjected to absorbance measurements at 220 nm to confirm the peptide concentration.
Fractions from the separation will be obtained by using a fraction collector
and antifungal and antibacterial activity will be carried out for all fractions against human pathogens.
The column was eluted with a gradient of ammonium acetate buffer (pH 5.2) at a flow rate of 5.0 mL/min and the sugar content was monitored by an evaporative light scattering detector with the eluate split 1 : 25 between the detector and the fraction collector
. Fractions were collected every min.
The Sepacor MPLC system (Biichi, Flawil, Switzerland) consisted of 2 pump modules C-605, pump manager C-615 and fraction collector
Constant time fractions (5 min/fraction) were collected with the help of a fraction collector
(Haake Buchler, model-100, USA).
Nanostream, which makes the Veloce micro parallel LC system, chose the ASMS meeting to announce a fraction collector
20-HETE and the internal standard eluted at a retention time of 6.9 min, and 1-min fractions were collected between 6.5 and 7.5 min with an automated fraction collector
They were the first to displace the column content and collect it in a fraction collector
. It proved to be difficult to obtain good separations because of the poor stabilization of the glass beads.