3A-Sham group; Negative Bcl 2 reaction ovarian folliculares and stromal area Figure 3B- Torsion group; An increase in Bcl 2 positive cells (arrow) Figure 3C-- Torsion-detorsion group, positive Bcl-2 expression in preantral, luteal folliculi
and interfollicular area (arrow) Figure 3D-Torsion-detorsion group + CAPE group, A reduction in the number of apoptotic cells in secondary follicules (red arrow) Bcl-2 immunostaining Bar 100 [micro]m
After removing the outer fibrous tissues and the separation of the granulosa layer, the remainder of each follicle (theca folliculi
) was inverted, and incubated for 30 min in Medium 199 containing 10 mM Hepes and 0.2% collagenase.
A layer of cells (Theca folliculi) was first appearing with spindle-shaped nucleoli and was enveloped of each oocyte (Fig.3, e).
Fig.(5, a) showed the hyperplastic atresia in the late vacuolated follicle which leads to the formation of pre-ovulatory corpus luteum that characterized by its stratified follicular epithelium and marked thickness with high vascularity of the theca folliculi. Fig.(5, b) illustrated artesian of oogonia with lipoid structures and atrophy of nucleus and cytoplasm.
folliculi, which shows host-associated differentiation (possibly cryptic speciation) on 2 species of goldenrod, is thought to have shifted from Solidago rugosa to S.
Genus species Accession number Dasineura carbonaria Felt EU375703 Dasineura folliculi Felt EU375702 Dasineura oxycoccana Johnson FR694171 Dasineura oxycoccana Johnson FR694172 Dasineura oxycoccana Johnson FR694174 Dasineura rosae Bremi AB505984 Dasineura strobila Dorchin EU375687 Dasineura tomentosa Dorchin EU375694 Table 2.
At necropsy, viscera samples (e.g., brain, heart, liver, spleen, lung, theca folliculi) were collected and placed in 10% buffered formalin.
RT-PCR was optimized by using the following controls, including nucleic acids extracted from theca folliculi of healthy Pekin ducks: avian influenza virus, Newcastle disease virus, egg drop syndrome virus, anatid herpesvirus 1, Muscovy duck parvovirus, goose parvovirus, duck reovirus, goose reovirus, duck hepatitis A virus, duck astrovirus, duck circovirus, and goose hemorrhagic polyomavirus.
Flavivirus-specific RNA was most frequently detected in theca folliculi, followed by intestinal mucosa, uterus, spleen, trachea, cloaca (swab), and liver (Table).
The flavivirus RNA was detected by NS3-based RT-PCR, and the virus was again isolated from theca folliculi.