folliculi, which shows host-associated differentiation (possibly cryptic speciation) on 2 species of goldenrod, is thought to have shifted from Solidago rugosa to S.
Genus species Accession number Dasineura carbonaria Felt EU375703 Dasineura folliculi Felt EU375702 Dasineura oxycoccana Johnson FR694171 Dasineura oxycoccana Johnson FR694172 Dasineura oxycoccana Johnson FR694174 Dasineura rosae Bremi AB505984 Dasineura strobila Dorchin EU375687 Dasineura tomentosa Dorchin EU375694 Table 2.
The theca folliculi from each duck were also used for virus isolation or detection by PCR.
RT-PCR was optimized by using the following controls, including nucleic acids extracted from theca folliculi of healthy Pekin ducks: avian influenza virus, Newcastle disease virus, egg drop syndrome virus, anatid herpesvirus 1, Muscovy duck parvovirus, goose parvovirus, duck reovirus, goose reovirus, duck hepatitis A virus, duck astrovirus, duck circovirus, and goose hemorrhagic polyomavirus.
Flavivirus-specific RNA was most frequently detected in theca folliculi, followed by intestinal mucosa, uterus, spleen, trachea, cloaca (swab), and liver (Table).
The flavivirus RNA was detected by NS3-based RT-PCR, and the virus was again isolated from theca folliculi.
Experimental infections further confirmed that TMUV can be reisolated from theca folliculi.