Given that fluorescein is the only fluorophore
used throughout the study, cytometric variations in the MESF values obtained are largely due to both procedure error and systematic error.
Once the subpopulations with the same color combination are selected, then the distance between fluorophores
The discussion below is based on the concept of fluorescence yield which is defined as the product of the fluorophore
concentration and the quantum yield of the fluorophore
A restricted class of molecules, fluorophores
, reach the ground state by emitting the energy as light at a wavelength equal to or longer than the wavelength absorbed.
may only fluoresce when excited by "invisible" UV light.
Four consecutive imaging series are performed in order to collect (1) RGB image at white LED illumination, helping to reveal hidden subcutaneous structures; (2) four spectral images at narrowband LED illumination for mapping of the main skin chromophores; (3) video-images under green LED illumination for mapping of skin blood perfusion; (4) autofluorescence video-images under UV LED irradiation for mapping of the skin fluorophores
Here we describe a magnetically prompted immunoreaction system with unique polymer-coated magnetic beads containing highly analytically sensitive fluorophores
In the case of a flow cytometer, the relative intensity of the fluorescence signal produced by a fluorophore
can be estimated by the fluorophore
's brightness index, BI, which is defined in Eq.
A 10-fold excess of the synthesized fluorophore
was incubated for 2 hours with an IgG antibody in basic pH followed by size-exclusion chromatography to obtain the pure product.
Thee new Brilliant Violet 421 products are part of the first commercial release of a novel, polymer-based fluorophore
The evolution of the concentration of a fluorophore
in a bead is described by a partial differential equation for the concentration c as a function of three spatial coordinates and time, c = c(x,y,z,t).
PG1, the mitochondrial membrane potential sensor JC-1, and the mitochondrial redox potential sensor MTroGFPl in living cells to characterize the role of oxidative stress in zinc-induced toxicity.