It was stated that DAPI may be preferred as the fluorochrome
for genome size estimation and PI was found to be less reliable (RAYBURN et al.
During the past few years, there have been significant changes regarding the qualifications for personnel credentialed to perform flow cytometry, assays with revived clinical applications, more elegant fluorochrome
development, and the size and sensitivity of instruments.
Bacterial cells was enumerated by epifluorescent microscopy (Axiosp 1, Zeiss, triple filter Texas Red-DAPI-fluorescein isothiocyanate, 1000x magnification) and using fluorochrome
fluorescein diacetate and UV-radiation (Kepner & Pratt, 1994).
Slides preparations were subjected to RNAse and Proteinase K pretreatments, followed by steps of denaturalization, probe hybridization, blocking, probe detection by means of antibodies linked to fluorochromes
[anti-digoxigenin to fluorescein (FITC) and anti-biotin to rhodamine (TRITC)] (Dako, USA), washing and DAPI staining for contrast.
Specific hybridization probes are probes labeled with two types of fluorochromes
, a donor and an acceptor.
the signal imbalances inherent in the fluorochromes
used tend to be more
Although immune cell imaging has been successfully performed with Fab fragments in mice [43, 44], their fluorochrome
binding is often less bright than that of classical antibodies because of their smaller size .
Whichever mode is applied, FPI is incapable of imaging deep fluorochromes
with high spatial resolution.
Stability of polylactic acid particles and release of fluorochromes
upon topical application on human skin explants.
BD CS&T beads are comprised of three separate beads that are hard-dyed with varying amounts of fluorescent molecules that mimic fluorochromes
that are excited by laser wavelengths of 355, 375, 445, 488, 532, 561, 633, 635, 640 nM.
This method allows users to control the emission of fluorochromes
, localizing the position of single molecules far below the diffraction limit.
PCR was performed in multiplex in a final volume of 15 L according to the following conditions: 1X STR 10X buffer (Promega Brazil); 1.5 mM MgCl2 (Promega Brazil); 1.4 pM each forward primer labeled with one of the fluorochromes
FAM HEX or TAMRA and 1.4 pM each reverse primer not labeled; 0.5 U Taq DNA Polymerase Platinum(R) (Life Technology Brazil) and 50-100 ng of genomic DNA.