The IHControls concentrations are listed in each figure legend, in units of molecules of equivalent fluorochrome
, or "MEF," per microbead (Materials and Methods).
(fluorescent dyes) are used in FC.
Purified monoclonal antibodies against HLA-DR molecules (clone L243) and its isotype control (IgG2a) were purchased from Ozyme (St Quentin en Yvelines, France) and covalently conjugated to fluorochromes
with FluoProbe 490 or 647 labeling kits (Interchim, Montlucon, France), via the -NHS ester.
As for the emission process, the source term Q is determined by the distribution of the photon density for excitation as well as the fluorescent yield of fluorochrome
a) antibody fluorochrome
complexes (eg Qdots, Alexa fluorochromes
, PE-based tandem fluorochromes
APC based tandem fluorochromes
etc.) In order to detect surface molecules and intracellular proteins simultaneously.
MSCs were harvested during the 4th passage and were labeled with PKH26, which is a red fluorochrome
. It has excitation (551nm) and emission (567 nm) characteristics compatible with rhodamine or phycoerythrin detection systems.
A variation of more than 20% in the mean fluorescent index for fluorochrome-labeled molecules of equivalent soluble fluorochrome
(MESF) calibration beads was observed for instruments that had been standardized using CS&T beads.
Molecular cytogenetics of Salminus fish (Characiformes) based on 5S and 18S rRNA genes hybridization, fluorochrome
staining and C-banding.
A histopathologic analysis should be performed using the fluorochrome
technique for optimal sensitivity.
To examine epithelial fields for evidence of apoptosis, cell sloughing, and "vacuole" loss, live animals were costained with 0.001% acridine orange, a fluorochrome
that binds nucleic acids, and 0.008% LysoTracker Red, a fluorochrome
that labels acidic compartments.
Samples were incubated with primary antibody either purified or conjugated with biotin or fluorochrome
for 1 h at room temperature.
The technique innovatively incorporates advantages of various methods and can be used in IgMs, fluorochrome
conjugates, immobilized antibodies and mixtures.