Vedadi, "The use of differential scanning
fluorimetry to detect ligand interactions that promote protein stability," Nature Protocols, vol.
Technique Use Advantages Disadvantages Fourier Chemical Fast and Complicated transformed composition inexpensive sample preparation infrared (FTIR) Characteristic Interference of each of water material Relatively low Possible sensitivity quantification Requires dried samples Destructive UV-Vis Quantification of Cost effective Interference concentration between Simple and fast materials Size and shape determination Useful for a variety of compounds
Fluorimetry Quantitative High sensitivity Limited to fluorescence Compound fluorescent determination specificity compounds Limited fluorescence lifetime Technique References Fourier Alben and transformed Fiamingo [27] Pretsch 2003 infrared Sapsford et al.
The lower rate of Pompe disease false positives using MS/MS vs
fluorimetry may be due to the relatively large analytical range of the MS/MS GAA assay (7).
At 30-minute intervals, the release of nile red was monitored using
fluorimetry in the same manner described above.
Serum angiotensin converting enzyme (ACE) activity was determined by indirect
fluorimetry according to methods described by Santos et al.
measured with through
fluorimetry. WTRs filter RMPs primarily through
Comparison of three methods for Chlorophyll determination: Spectrophotometry and
Fluorimetry in samples containing pigment mixtures and spectrophotometry in samples with separate pigments through High Performance Liquid Chromatography.
The research group used a method known as high-throughput differential scanning
fluorimetry, or HT-DSF, in which proteins emit light when they reach their melting point.
Use of real-time PCR and
fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis.
We evaluated the effects of lichen extracts on caspase-3 activity by
fluorimetry. As shown in Table 3, exposure of U373-MG cells to [H.sub.2][O.sub.2] produced a remarkable increase of over 300% in caspase-3 activity relative to control cells.
The extraction of the photosynthetic pigment was made in 90% acetone (Merck[R] PA) in the dark at -18[degrees]C for 24 hours, for later determination of chlorophyll-a concentration by
fluorimetry (Strickland and Parsons 1972).
Fluorimetry as a simple and sensitive method for determination of catalase activity.
