Fluorescence lifetime measurements were performed with an Edinburgh Instruments OB 900 time-correlated single-photon counting
fluorimeter. The excitation of the samples was carried out with a PicoQuant PLS450 diode with emission centered at ~450 nm and pulse FWHM of 0.8 ns.
The intense blue color formed as a result of reduction to the ferrous-tripyridyltriazine complex was measured at 593 nm (Spectramax Gemini EM
fluorimeter, Molecular Devices, Sunnyvale, USA).
The fluorescence efficiency rates were measured using a Handy Plant Efficiency Analyser fluorometer (Handy PEA
fluorimeter; Hansatech Instruments Ltd., King's Lynn, UK).
At each specified time point, the fluorescence of the solution was measured (excitation 492 nm, emission 535 nm) using a Wallac 1420 Victor 3 96-well plate reader (PerkinElmer, USA)
fluorimeter and plotted as a function of time with Origin[R]7 (OriginLab Corporation, Northampton, USA).
After incubation, AAPH (12 mM) was added and fluorescence was recorded for 98 min at excitation and emission wavelengths of 485 nm and emission of 520 nm, respectively, in a FLUOstar Optima
fluorimeter (BMG Labtech, Ortenberg, Germany).
Tambem aos 90 dias, foram determinados o rendimento quantico maximo do fotossistema II ou eficiencia fotoquimica (Fv/Fm) e o indice fotossintetico (PI), com auxilio do fluorimetro nao modulado, modelo Pocket PEA Chorophyll
Fluorimeter (Hansatech Instruments--King's Lynn, Norfolk).
The LightCycler: a microvolume multismaple
fluorimeter with rapid temperature control.
Fluorescence measurements were done on attached leaf blades using a portable
fluorimeter (Opti-Science 30).
The detection of amplified EBOV DNA fragment is performed in
fluorimeter channel FAM with the fluorescent quencher BHQ1.
The identification of amplified EBOV DNA fragment is done in
fluorimeter channel FAM with the fluorescent quencher BHQ1.
Mahmood, "Development of a time domain
fluorimeter for fluorescent lifetime multiplexing analysis," IEEE Transactions on Biomedical Circuits and Systems, Vol.
The
fluorimeter was configured to use fixed excitation and emission wavelengths at 488 and 660 nm, respectively.