Kinyoun staining was positive in 40 (41%) and fluorescent stain
was positive in 40 (41%) cases.
Ziehl-Neelsen (ZN) stain is used worldwide as a standard method to detect AFB, but it has less sensitivity relative to fluorescent stain
as it takes more time to scan at least 300 fields and often misses the paucibacillary tuberculosis.
The green fluorescent stain
(SYTO 9, with excitation/emission maxima of about 480/500 nm) colors healthy bacteria that have intact membranes, thus staining live cells; and the red dye (propidium iodide with excitation/emission maxima of about 490/635 nm) colors bacteria with damaged membranes, by displacing the green dye, thus staining dead cells.
After 5 d of in vitro culture, blastocyst rates were evaluated based on the number of embryos used for culture and were subsequently stained with Hoechst 33342 fluorescent stain
to count their total cell number.
trachomatis-LPS using a nuclear supravital fluorescent stain
(Hoechst 33258) which enters the cell nucleus and binds DNA only when the plasmamembrane is damaged.
SPERM VIABILITY DETECTION BY FLOW CYTOMETRY WITH FLUORESCENT STAIN
With a monoclonal antibody fluorescent stain
(Meridian Diagnostics, Cincinnatti, OH), Cryptosporidium sp.
First of all, ZN stain has low sensitivity relative to fluorescent stain
 and culture , secondly it takes more time to scan at least 300 field.
The acridine orange fluorescent stain
I use works fine on our fluorescent microscope.
The D3 Duet(TM) DFA kits use brighter, easily distinguishable fluorescent stain
colors to improve interpretation of virus-infected cell cultures by combining monoclonal antibodies labeled with either Fluorescein Isothiocyanate (FITC) or R-Phycoerythrin (R-PE) in one reagent for simultaneous detection.
Prepare phenolic acidine orange fluorescent stain
for mycobacteria as shown in Figure 4.
Staining methods like ZN and fluorescent stains
are rapid but lack the sensitivity, whereas culture is highly sensitive but is time consuming.