fluorescence-activated cell sorting


Also found in: Dictionary.
Related to fluorescence-activated cell sorting: cytometry, FACS

fluorescence-activated cell sorting

Abbreviation: FACS
A method of separating cells by selectively tagging them with colored fluorescent dyes bound to specific cellular structures or molecules.
See also: cell sorting
Mentioned in ?
References in periodicals archive ?
At the same time, we examine the effect of Grail on stress-induced apoptosis and tumorigenesis using fluorescence-activated cell sorting (FACS) analysis and an anchor-independent assay, respectively.
(A) Fluorescence-activated cell sorting (FACS) of GFP-positive cells after co-transfection of the Cas9-D10A nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs).
Positive clones were enriched using fluorescence-activated cell sorting and a FITC-labelled anti-ERBB2 monoclonal antibody (Abcam, Cambridge, UK).
(b) (1) Fluorescence-activated cell sorting (FACS) analysis was used to assess the accumulation of gH2AX foci in haMSCs.
Called SIF-seq, for site-specific integration fluorescence-activated cell sorting followed by sequencing, this new technique developed by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) complements existing genomic tools, such as ChIP-seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.
Enrichment of target cells, for example, by fluorescence-activated cell sorting (FACS), is restricted to cases where a leukemia-associated aberrant immune phenotype (LAIP) is available [13].
After washing, cells were analyzed on a fluorescence-activated cell sorting (FACS) arial flow cytometer with CellDevia software (BD Biosciences, USA).
The topics include purifying and cultivating retinal ganglion cells from rodents, purifying dorsal root ganglion neurons from rats by immunopanning, purifying astrocytes from transgenic rodents by fluorescence-activated cell sorting, myelinating co-cultures of rat retinal ganglion cell re-aggregates and optic nerve oligodendrocyte precursor cells, and purifying Schwann cells from the neonatal and injured adult mouse peripheral nerve.
Fluorescence-activated cell sorting analysis: FACS flow cytometry analysis of cell cycle and apoptosis was performed using our previously described method.1314 In brief Saos-2 cells were seeded in six-well plates (1A-106 cells/well of Saos-2).
Utilizing fluorescence-activated cell sorting (FACS), a sample of mixed skeletal muscle can be sorted by the cells expressing [alpha]7 for myoblast enrichment [12,13].
LifeSpan's catalogue of 93,000 antibodies are used for research applications including immunoblotting, immunohistochemistry, immunofluorescence, and fluorescence-activated cell sorting. LifeSpan also provides comprehensive services, including antibody synthesis, peptide design, antibody purification, immunohistochemistry and pathology interpretation, to assist customers in their research, diagnostics and drug development needs.
Fluorescence-activated cell sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M.

Full browser ?