fluorescence quenching


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fluor·es·cence quench·ing

a technique used in investigations dealing with binding of antigens (haptens) by purified antibodies, applicable in cases in which the bound antigen (hapten) absorbs (quenches) light emitted during fluorescence of protein (antibody) excited by ultraviolet light.

fluorescence quenching

A technique for investigating antigen-antibody reactions by measuring the light absorbed by an antigen mixed with a fluorescent-labeled antibody.
See also: quenching
References in periodicals archive ?
For the dynamic fluorescence quenching, its binding constant (KA) and the number of binding site (n) could be obtained by using the following Lineweaver-Burk equation:
When the pH value increased in the tested range, diminutive change in the fluorescence intensity of AuNCs-BSA was observed, whereas the fluorescence intensity of the AuNCs-BSA in the presence of [Cu.sup.2+] increased, indicating the fluorescence quenching efficiency of [Cu.sup.2+] decreased with increasing of the pH value.
Caption: Figure 9: Dependence of fluorescence quenching of gcDNA bound YOYO with varying dye to DNA molar ratio in the presence of increasing [alpha]-PrP concentrations.
This study analyzed the interaction between the novel patent organotin compound DBDF2,6T and the hPPAR[gamma] protein under physiological condition with the methods of fluorescence quenching, 3D fluorescence, DARTS, ultrafiltration-LC, and computer molecular docking.
ANS fluorescence quenching is also cheaper; nevertheless, it is laborious and time-demanding for HTS screening, beyond the fact that it is an in vitro approach [36, 39].
Caption: Figure 1: Diagram of test strip used in the background fluorescence quenching immunochromatographic assay (bFQICA).
Fluorescence quenching constant (KSV) values were given as means [+ or -] standard deviation and values of disulfide reductase activity were expressed in % of control sample [+ or -] relative standard deviation.
These changes in the fluorescence characteristics (fluorescence quenching and blue shift in the emission maxima) observed in the presence of 6-shogaol were suggestive of tertiary structural alteration in the protein induced by 6-shogaol binding.
However, these small particles must be functionalized to avoid agglomeration and fluorescence quenching. Their potential for chemical and biological sensing is largely unexplored.
The electronic and optoelectronic properties of semiconductor quantum dots (QDs) are mediated by surface defects due to the presence of dangling bonds producing trap states within the HOMO-LUMO energy gap, and contributing to fluorescence quenching. Surface capping ligands are generally used to alleviate this problem and increase the quantum yields of QDs.
Diurnal variations in gas exchange chlorophyll fluorescence quenching and light allocation in soybean leaves: The cause for midday depression in CO2 assimilation.
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