fluorescence polarization immunoassay


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Related to fluorescence polarization immunoassay: Cloned Enzyme Donor Immunoassay

fluorescence polarization immunoassay

, fluorescent polarization immunoassay Abbreviation: FPIA
A means of identifying and quantifying the amount of an antigen in a specimen in which a fluorescently labeled antibody is mixed with a sample thought to contain the antigen is exposed to polarized light. Bound fluorescently labeled antibody reacts to polarized light in a characteristic fashion not demonstrated by unbound antibody, allowing for identification and measurement of the ligand.
See also: immunoassay
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Design.--Aliquots of drug-free serum pools were supplemented with ginseng and apparent digoxin concentrations were measured using enzyme-linked chemiluminescent immunosorbent digoxin assay, turbidimetric assay, and fluorescence polarization immunoassay digoxin assays.
Most drugs are currently monitored by ligand assay techniques, particularly enzyme and fluorescence polarization immunoassays.
Fluorescence polarization immunoassay. In: Wolfbeis OS, ed.
Louis, MO), Beckman enzyme immunoassay (EIA) reagents on a Synchron[R] CX-9 (Children s Mercy Hospital, Kansas City, MO), or fluorescence polarization immunoassay (FPIA) technology on an Abbott Ax5YN1[R] (Children s Hospital of Wisconsin, Milwaukee, WI) according to the manufacturer's instructions in each case.
For carbamazepine, the intralaboratory variance of the Syva Emit was significantly greater than that for the two Abbott methods, whereas the interlaboratory variance of the CEDIA method was significantly greater than that for the Abbott AxSYM, Roche fluorescence polarization immunoassay (FPIA), and HPLC.
Immunologic screening of the urine samples was performed with two different immunoassays: the automated AxSYM analyzer (Abbott) with the Abbott fluorescence polarization immunoassay (FPIA) Amphetamine/Methamphetamine II and the Mahsan-AM[P.sub.300] on-site test (Mahsan Diagnostika).
The following digoxin methods were performed as suggested by the manufacturers: AxSYM MEIA II; IMx[R] MEIA II (singleton analyses; Abbott); TDx[R] fluorescence polarization immunoassay (FPIA; singleton analyses; Abbott); Emit 2000[R] (bade Behring) on the Cobas Mira 5 system; Dimension[R] (bade Behring); aca[R] (bade Behring) on the aca SX (singleton analyses); TinaQuant[R] (Roche) on the Hitachi 912; Elecsys[R] (Roche) on the Elecsys 2020; and Vitros[R] slides (Ortho Clinical Diagnostics) on the Vitros 950.

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