fluorescence microscopy


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fluorescence

 [floo͡-res´ens]
the property of emitting light while exposed to light, the wavelength of the emitted light being longer than that of the absorbed light.
fluorescence microscopy the use of a fluorescence microscope to identify microorganisms or specific tissue constituents that have been stained with a fluorochrome or a fluorochrome-labeled substance (such as an antibody to a tissue antigen). A fluorescent antibody test can be used in place of time-consuming culture methods for identifying bacteria. See also immunofluorescence.

microscopy

 [mi-kros´kah-pe]
examination with a microscope.
fluorescence microscopy conjugation of antibodies with fluorescent dyes in order to identify specific microorganisms or tissue constituents; see also fluorescence microscopy.

fluor·es·cence mi·cros·co·py

a procedure based on the fact that fluorescent materials emit visible light when they are irradiated with ultraviolet or violet-blue visible rays; some materials manifest this property naturally, whereas others may be treated with fluorescent solutions (somewhat analogous to staining); when the absorption of the specimen is in the relatively long ultraviolet range a filter that transmits these radiations is used, and a yellow filter is placed on or in the ocular; the background field is then dark, and any yellow or red fluorescence becomes visible.

fluorescence microscopy

n.
Microscopy using naturally fluorescent or treated materials that emit visible light when they are irradiated with ultraviolet or violet-blue visible rays.

fluor·es·cence mi·cros·co·py

(flōr-es'ĕns mī-kros'kŏ-pē)
A procedure based on the fact that fluorescent materials emit visible light when they are irradiated with ultraviolet or violet-blue visible rays; some materials manifest this property naturally, whereas others may be treated with fluorescent solutions (somewhat analogous to staining).

fluorescence microscopy

a method of microscopy in which fluorescent dyes are used to mark certain structures (e.g. nucleic acids) which can then be viewed with an ultraviolet microscope.
References in periodicals archive ?
The scope of this report covers the global fluorescence microscopy market for various end-user application industries.
Grayscale (A, D, G, and J) and digitally pseudocolored (B, E, H, and K) confocal fluorescence microscopy images along with their corresponding hematoxylin-eosin-stained tissue sections (C, F, I, and L) of correctly recognized invasive ductal carcinoma of the breast; renal cell carcinoma, clear cell type; squamous cell carcinoma of the lung; and adenocarcinoma in the liver, respectively (original magnifications X100 [A through F] and X40 [G through L]).
High-speed 2D and 3D fluorescence microscopy of cardiac myocytes.
LED fluorescence microscopy has demonstrated increased sensitivity and speed in numerous research and clinical applications, including the detection of infectious diseases such as tuberculosis and malaria.
This work follows at least a decade of research building on the nearly 50-year history of fluorescence microscopy. The technology has been a multidisciplinary springboard of optical engineering, chemistry, and biology, in which the disciplines all have contributed to visualizing fluorescent dyes attached to proteins, advancing our understanding of cellular activity.
Washington, May 5 (ANI): A team of scientists at the University of Georgia (UGA), US, has developed a microscope that is capable of live imaging at double the resolution of fluorescence microscopy using structured illumination.
Agilent has agreed to acquire TILL Photonics GmbH, a provider of fluorescence microscopy products.
The toolbox supports QImaging and Hamamatsu cameras used in fields such as fluorescence microscopy and particle image velocimetry, as well as hardware from DALSA (formerly Coreco), Data Translation and Matrox Imaging.
"Fluorescence microscopy, specifically FISH, allows the direct localization of DNA and RNA sequences on chromosomes, in cells, and in tissue.
Another marker for apoptosis, annexin V, was used to evaluate membrane changes with fluorescence microscopy and flow cytometry.
This module is intended to familiarize the trainee with the use of the fluorescence microscope and fluorescence microscopy techniques.
The product's broad brand xenon spectrum is also an appropriate "tunable" source for narrow band illumination applications such as fluorescence microscopy, PerkinElmer, Inc.

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