FLOT1

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FLOT1

A gene on chromosome 6p21.3 that encodes a scaffolding protein present in caveolar membranes that participates in formation of caveolae or caveolae-like vesicles.
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References in periodicals archive ?
NTA revealed that the ADSC-derived exosomes appeared as nanoparticles (40-200 nm), and Western blotting confirmed positive expression of CD9, CD81, flotillin-1, and HSP70 versus ADSC cytoplasmic proteins (all P < 0.01).
For Western blotting analysis of the culture medium, conditioned medium, and exosome fractions, the following primary antibodies were used: mouse monoclonal anti-CD9 (Santa Cruz Biotechnology, USA), rabbit polyclonal anti-CD81 (TSG101; Abcam, USA), flotillin-1 (Abcam), and HSP70 (Abcam) antibodies.
Western blotting analysis confirmed the expression of CD9 ( t = −295.483, P < 0.01), CD81 ( t = −304.686, P < 0.01), flotillin-1 ( t = −158.569, P < 0.01), and HSP70 ( t = −175.036, P < 0.01) in the exosome fractions, compared with the ADSC cytoplasmic proteins [Figure 6]a and [Figure 6]b.{Figure 5}{Figure 6}
Galbiati, "Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells," FASEB Journal, vol.
This substance is essential for inhibiting the maturation of the bacterial vacuole via preventing cholesterol-rich lipid rafts which are characterized by their enrichment in flotillin-1 and consequently prevent lysosome fusion (Dermine et al., 2001).
In contrast, Annexin V, TSG101, and Flotillin-1 could not be detected on the leukocyte-derived sEVs.
Furthermore, TNF RI, TLR3, CD42a, CD80, and CD83 as well as Annexin V, Flotillin-1, and Alix were detected on the majority of the plasma sEVs.
Immunological markers Functional Regulatory Adhesion Lineage-specific and others HLA-DR CTLA-4 CD11a CD3 HLA-ABC PD-L1 ICAM-1 CD4 CD28 CD8 CD80 CD14 CD49d CD16 TLR3 CD19 Fas-L CD37 MIC A/B CD41 TNF RI CD42a TNF RII CD45 CD56 CD83 Vesicle-related markers Functional Tetraspanins Others HLA-DR CD9 TSG101 HLA-ABC CD63 Alix CD28 CD81 Flotillin-1 CD80 CD82 Annexin V CD49d TLR3 Fas-L MIC A/B TNF RI TNF RII HLA: human leukocyte antigen; TLR: Toll-like receptor; TNF: tumor necrosis factor: RI: receptor I, RII: receptor II; MIC A-B: major 1; TSG101: tumor susceptibility gene 101.
The results of an in vitro study on Tangier fibroblasts indicate that ABCA1 forms a complex with syntaxin 13 and flotillin-1, which resides the plasma membrane and phagosomes partially located in raft microdomains [92].
Boettcher et al., "Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells," Molecular Biology of the Cell, vol.
Furthermore, immunoprecipitation assays were performed for the detection of flotillin-1, which is a protein that is typically found in lipid raffs [20], Figure 3(b) shows that, in macrophages that have ingested apoptotic cells, the amount of flotillin-1 was significantly increased in the samples immunoprecipitated with antibodies to CD36.
In addition, we showed that efferocytosis increased the colocalisation of these receptors and immunoprecipitation with the membrane lipid raft marker protein flotillin-1; also we demonstrated that lipid raft integrity is required for the optimal ingestion of AC.