Each group of 10 mice (5 males and 5 females) received, respectively, a single oral dose of 10, 15, 20, 25, 30, 40 or 50 ml/kg body weight of Nigella sativa fixed oil. Seventy other mice divided into 7 groups were used to study toxicity intraperitoneally.
The control group rats (C-rats) and Nigella sativa-treated rat group (Ns-rats) received, respectively, 2 ml/kg body weight of distilled water and Nigella sativa fixed oil via the oral route for 12 weeks.
The fixed oil obtained from Nigella sativa seeds (chestnut color, agreeable perfume, extraction yield: 26% w/w) was analyzed using thin layer chromatography (TLC).
The current study has shown a low toxicity of Nigella sativa fixed oil. The high values of oral and intraperitoneal lethal doses of Nigella sativa fixed oil ([LD.sub.50] value = 28.8 ml/kg body wt., p.o.
Nigella sativa fixed oil has been well-analyzed (Abdel-Aal and Attia, 1993; Aboul-Enein et al.
Nigella sativa fixed oil, like the fibrates, decreases serum cholesterol and triglycerides, and ameliorates serum HDL.
Nigella sativa fixed oil exhibits potent analgesic and sedative properties (Khanna et al.
Further research is needed to elucidate the precise mechanisms of different actions of Nigella sativa fixed oil.
The present study was carried out to examine the antioxidant, antimicrobial and cytotoxicity potential Malcolmia africana leaves extract, fraction, essential oil and fixed oil. The whole plant was extracted with absolute methanol and further fractionated with increasing polarity based absolute solvents.
Essential oil of leaves showed less IC50 value and % inhibition than fixed oil. Results are shown in Table-12.
Table-12: Antioxidant potential of essential oil and fixed oil Malcolmia Africana leaves.
The fixed oil of leaves exhibited good results against bacteria but did not give good results against fungal strains (Tables13-16).