siRNAs targeting the human Filamin B (Filamin B siRNA: sc-60641; Santa Cruz, CA, USA) the Aminopeptidase N (CD13 siRNA: sc-29960; Santa Cruz), and a scrambled (siRNA-A control: sc-37007; Santa Cruz) mRNAs, were used to transfected BM-MSC cultures by means of Lipofectamine 2000 (Invitrogen, CA, USA) according to the manufacturer's protocol.
Among the eleven identified proteins, we selected the Filamin B and CD13 to evaluate whether they could be involved in the key functions of senescent BM-MSCs.
These results suggest that Filamin B is not involved in the determination of primary (stress induced) senescence whereas its presence could be part of the mechanism that regulate the paracrine senescence induction in human BM-MSCs.
Filamin B is an actin-binding protein which crosslinks actin cytoskeleton filaments into a dynamic structure.
Differential expression of filamin B splice variants in giant cell tumor cells.
Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices.
Targeting filamin B induces tumor growth and metastasis viaenhanced activity of matrix metalloproteinase-9 and secretion of VEGF-A.
Filamin B and CD13 Are Components of Senescent Secretomes That May Be Involved in Primary (Stress Induced) and Paracrine Senescence of Mesenchymal Stromal Cells.