Another potential cause for Ube3A-related neurodevelopmental dysfunction is that Ube3A also targets Ephexin5, an activator of the small GTPase RhoA, which is associated with the contraction of
filamentous actin networks [91].
Selective assay of monomeric and
filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.
F-actin pre acrosomal reaction denotes the amount of
filamentous actin present in unreacted sperm visualized by fluorescent staining and electron microscopy.
If this were an art show, viewers would be struck by the otherworldy beauty and dumbstruck by the subjects: for instance,
filamentous actin and microtubules in mouse fibroblasts.
Once the microbe has escaped from the vacuole, it quickly becomes coated with
filamentous actin and ultimately forms an actin tail at one pole of the bacterium (Figure 3A) (46,47).
Confocal microscopy images of F-actin stained with Phalloidin showed that, in PEG 35 pretreated livers,
filamentous actin associated with the membrane microfilamentous network and the pericanalicular band are kept preserved (Figure 5(a)).