QRT-PCR analysis indicated the transcription of the selected lncRNAs: RP11-517C16.2-001, FR271872, LOC283352, RP11-401E9.3, FGFR3P, XXbac-BPG16N22.5, and mRNAs: collagen 19a1 (COL19A1), nitric oxide synthase 2 (NOS2), chromosome 13 open reading frame 15 (C13orf15), FBJ murine osteosarcoma viral oncogene homolog (FOS), ficolin
(collagen/fibrinogen domain containing lectin) 2 (hucolin) (FCN2), serine peptidase inhibitor, Kunitz type 1 (SPINT1), placenta-specific 8 (PLAC8), E2F transcription factor 1 (E2F1), and baculoviral IAP repeat containing 5 (BIRC5), perfectly correlated to microarray results.
. Three members of the ficolin
family of proteins have been described in humans, such as L-ficolin (or ficolin-2), H-ficolin (or ficolin-3 or Hakata antigen), and M-ficolin (or ficolin-1) [16-19].
LP is activated by binding of mannose binding lectin (MBL) or ficolin
to pathogen-associated molecular patterns (PAMPs) on the surface of microbes.
After blocking the endogenous peroxidase with 30 g/L hydrogen peroxide, for ficolin
staining alone, sections were incubated in TBS with normal sheep serum, with either H-ficolin monoclonal antibody (clone 4H5) from HyCult Biotechnology or rabbit polyclonal anti-L-ficolin antibody (clone 2F5) from Tokai University.
The lectin pathway can be initiated by the collectins MBL, CL-K1, and CL-LK and all the ficolins
Thiel, "Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins
and associated proteins," Molecular Immunology, vol.
Collections and ficolins
: humoral lectins of the innate immune defense.
Just in mammalian species calreticulin was reported as a receptor for C1q mannose-binding lectins and ficolins
Innate immunity consists of a highly regulated immune surveillance system comprising several humoral factors, including soluble PRRs, such as collectins, ficolins
, and pentraxins [32, 33], and the complement cascade .
Conversely, particles coated with poloxamine in the mushroom-brush and fully brush conformation activate the complement via the lectin pathway, which involves the opsonisation of mannose-binding lectin protein (MBL) and/or ficolins
. This complement activation pathway was attributed to the structural similarities between the EO monomers of poloxamine and a region of D-mannose .