QRT-PCR analysis indicated the transcription of the selected lncRNAs: RP11-517C16.2-001, FR271872, LOC283352, RP11-401E9.3, FGFR3P, XXbac-BPG16N22.5, and mRNAs: collagen 19a1 (COL19A1), nitric oxide synthase 2 (NOS2), chromosome 13 open reading frame 15 (C13orf15), FBJ murine osteosarcoma viral oncogene homolog (FOS), ficolin
(collagen/fibrinogen domain containing lectin) 2 (hucolin) (FCN2), serine peptidase inhibitor, Kunitz type 1 (SPINT1), placenta-specific 8 (PLAC8), E2F transcription factor 1 (E2F1), and baculoviral IAP repeat containing 5 (BIRC5), perfectly correlated to microarray results.
. Three members of the ficolin
family of proteins have been described in humans, such as L-ficolin (or ficolin-2), H-ficolin (or ficolin-3 or Hakata antigen), and M-ficolin (or ficolin-1) [16-19].
Innate immune response by ficolin
binding in apoptotic placenta is associated with the clinical syndrome of preeclampsia.
LP is activated by binding of mannose binding lectin (MBL) or ficolin
to pathogen-associated molecular patterns (PAMPs) on the surface of microbes.
After blocking the endogenous peroxidase with 30 g/L hydrogen peroxide, for ficolin
staining alone, sections were incubated in TBS with normal sheep serum, with either H-ficolin monoclonal antibody (clone 4H5) from HyCult Biotechnology or rabbit polyclonal anti-L-ficolin antibody (clone 2F5) from Tokai University.
The lectin pathway can be initiated by the collectins MBL, CL-K1, and CL-LK and all the ficolins
Thiel, "Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins
and associated proteins," Molecular Immunology, vol.
These include complement components, collectins, ficolins
, and pentraxins.
Collections and ficolins
: humoral lectins of the innate immune defense.
Just in mammalian species calreticulin was reported as a receptor for C1q mannose-binding lectins and ficolins
Innate immunity consists of a highly regulated immune surveillance system comprising several humoral factors, including soluble PRRs, such as collectins, ficolins
, and pentraxins [32, 33], and the complement cascade .
Conversely, particles coated with poloxamine in the mushroom-brush and fully brush conformation activate the complement via the lectin pathway, which involves the opsonisation of mannose-binding lectin protein (MBL) and/or ficolins
. This complement activation pathway was attributed to the structural similarities between the EO monomers of poloxamine and a region of D-mannose .