Commonly used additives in the maintenance cell culture medium are fetal calf
serum (FCS), goat serum, horse serum, allontoic fluid, colostral whey, oval albumin, etc.
In the past, the islets have all been isolated with fetal calf
Although bovine sera of alternative origin are available, like newborn calf serum, calf serum, and donor bovine serum, fetal bovine (fetal calf
) serum (FBS/FCS) is most widely used due to its low content of immunoglobulins and complement factors (Gstraunthaler and Lindl, 2013).
Three types of maturation media (MM) supplemented with 1) 10% fetal calf
serum (FCS), 2) 6 mg/ml FAF-BSA or 3) 6 mg/ml FAF-BSA + 10.0 ng/mL epidermal growth factor (EGF) were evaluated.
Using Zika virus stock containing 2.5% fetal calf
serum (FCS) mixed 3:10 (vol/vol) with indicated alcohols, we incubated the mixture for 1 minute and then used it for infection (Figure, panel A).
Primary antibodies were diluted 1:100 in 10% fetal calf
serum and applied to the spot for 1 hr at room temperature.
HEK-293 cells (22) (ATCC number CRL-1573) were cultivated in DMEM supplemented with 100 mL/L fetal calf
serum, 10 kIU/L penicillin, and 100 mg/L streptomycin.
Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf
HT1080 cells were grown in supplemented DME medium containing glutamine (1 mM), penicillin (100 IU/ml), streptomycin, (100 [micro]g/ml), and 10% heatinactivated fetal calf
Belot and colleagues remind us that in vitro tests often still involve the use of fetal calf
serum or other products of animal origin.
The other half of the tick was homogenized in inoculation media (Dulbecco modified Eagle medium, 10X penicillin/streptomycin, 2% fetal calf
serum, and 20 mM HEPES buffer) by FastPrep-24 (MP Biomedicals, Santa Ana, CA, USA) using lysing matrix A.
The virus was propagated on monolayer of BHK-21 cell line in Roux flasks containing 100 ml of maintenance medium with 1 % fetal calf
serum (Muhammad et al., 2011).