The strains of Aspergillusniger have higher capability of producing calcium gluconate than other strains of microorganisms(Kundu and Das, 1982; Kaczmarowicz and Walisch, 1983, Baajaree and Kannika, 1993).The aim of the work was to produce calcium gluconate by using a strain of Aspergillusniger in both shake flasks and stirred fermentor
using glucose salt CaCO3 medium.
Key words: Kluyveromyces Marxianus, Fermentor
, Ethanol, Molasses, Nitrogen, Renewable energy.
was inoculated with 170 ml of "pellets" previously obtained in M1 Erlenmeyer flasks.
Medium consisted of 1.5 L of orange peel extract, supplemented with nitrogen source at its optimum concentration and salts 1% of K[H.sub.2]P[O.sub.4] and 0.5% MgSO4 in a 3 L Stirred tank fermentor
(New Brunswick, Bioflo 110) with six bladed Rushton turbine.
: Come to think of it, I've seen them flinching a bunch of times.
--unit for control of rotation of motor M1 (for mixing of liquid into fermentor
Immediately, I noticed the three custom-made fermentors
. Made of gleaming stainless steel, each fermentor
has a 143-gallon capacity.
This is true throughout the process, but especially critical at the point where sterile wort is introduced to the seed fermentor
. To optimize process efficiency, Fleischmann's has been able to determine optimal wort levels and minimize alcohol buildup.
The cassava starch used in this study was from the local cassava starch company in Thailand, and two commercial enzymes amylase (GC147) and glucoamylase (GC358; Genencor, Danisco US Inc., USA) were used to hydrolyze a 50 % W/V solution of cassava starch in a 4-L (2 kg of cassava starch: 2 L of distilled water) working volume in a 5-L of fermentor
. A volume of 400 [micro]L of GC147 was added to 2 L of preheated distilled water (90 [degrees]C), incubated for 4 h at 85 [degrees]C and agitated at a speed of 300 rpm.
Batch cultures of the lipase production were carried out in a 5-liter fermentor
(Bioengineering) with a working volume of 3 liter.
After a few hours in a deep-tank fermentor
, the bacteria can convert 72 percent of the sugar into mannitol.