Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints
: an animal model of osteoarthritis.
Kilgore, "Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis," Toxicologic Pathology, vol.
At necropsy, animals were asphyxiated with CO2 and the femorotibial joint was carefully dissected out, fixed in 10% buffered formalin, and sent to Nationwide Histology (Veradale, WA, USA) for further histopathology analysis.
The model involves inoculation of MIA into a femorotibial joint pocket that induces pain responses in the ipsilateral limb accompanied by progressive cartilage degradation.
The inclusion criteria were as follows: Patients with PFOA, mainly from patella wear and osteophytosis only; intact femorotibial joints, meniscus, cruciate ligaments, and collateral ligaments; and normal lower-limb alignment and normal bending and stretching abilities.
Local blocking of the patellofemoral joint was tested among patients whose femorotibial joint, meniscus, cruciate ligaments and collateral ligaments were all intact.
Histological lesion scoring: Rats from treated un-treated and control (normal) were sacrificed under high dose of anesthetic ether on 60th day and femorotibial joints were separated.
Papain induced degenerative changes in articular cartilage of rat femorotibial joint and its histopathological grading.
After blood collection, the animals were euthanized using [CO.sub.2] gas in special device and the MIA- and saline-treated femorotibial joints
were dissected and half of the samples (4 animals) were fixed in 10% formalin for further pathological examinations and the second half of the tissues (4 animals) were snap frozen in liquid nitrogen and then immediately were stored at -70[degrees]C for further biochemical and molecular analyses.
Femorotibial joints of rats were separated with sharp knife on each sampling day.
Progressive chronic osteoarthritis in femorotibial joints of partial medial meniscectomized guinea pigs.