feeder layer


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feeder layer

A population of connective tissue cells that are used to nourish cultured tissue cells in the laboratory. The feeder cell layer is often derived from mouse fibroblasts. Feeder cells supply metabolites to the cells they support, do not grow or divide, and can be inactivated by gamma irradiation.
See also: layer
References in periodicals archive ?
Yamato et al., "Human adipose tissue-derived mesenchymal stem cells as a novel feeder layer for epithelial cells," Journal of Tissue Engineering and Regenerative Medicine, vol.
del Rio, "Feeder layer cell actions and applications," Tissue Engineering.
Suzuki et al., "Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells," Stem Cells, vol.
Generating safer and more efficient cells using more appropriate culture media and feeder layers provides more adequate cells for cell transplantation therapies.
Vigorously pipetted up and down to obtain single cells from plucked hair, and then centrifuged at 200xg for 5 them for specific period of time in serum based medium without feeder layer.
We now know that standardization of the method with human originated feeder layer cells and use of animal-free medium are necessary to use these cells in therapeutic applications.
Previous studies have shown that human limbal epithelial progenitor cells can be expanded and maintained on intact, but not denuded HAM or plastic in the absence of 3T3 murine fibroblast feeder layers. Intrestingly He et al (22) has demonstrated the role of PI3K/Akt signaling pathways on the cells expanded over the intact membrane.
Presently, appropriate cytokines and feeder layers are widely applied to keep cESCs undifferentiated.
The results showed that some keratinocytes in monolayer cultures highly expressed C4.4A at cell-cell borders (Figures 1(a)-1(b), arrows), while the murine 3T3 fibroblasts, used as a feeder layer in the culture, were devoid of reactivity (Figure 1(a), arrowhead).
After electroporation, mESCs were allowed to spread on pre-coated gelatin-plastic flasks containing mitomycin C-inactivated feeder layer of primary cultures of mouse embryonic fibroblasts (MEF) in ESC medium containing G418 (Sigma, USA).
(connexin 43 and keratin 3 / keratin 12) on the limbal epithelial cells cultured over the denuded,AM with and without the 3T3 murine fibroblast cells as feeder layer.
Briefly, mitomycin c- (MMC- ; Sigma-Aldrich) treated (20 [micro]g/ml, for 3 h) mouse bone marrow stromal cells (M210B4; Korean Cell Line Bank, Seoul, South Korea) were seeded at 3 x [10.sup.5] cells/well in a 35 mm culture dish as a feeder layer the day before the assay.