feeder layer


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feeder layer

A population of connective tissue cells that are used to nourish cultured tissue cells in the laboratory. The feeder cell layer is often derived from mouse fibroblasts. Feeder cells supply metabolites to the cells they support, do not grow or divide, and can be inactivated by gamma irradiation.
See also: layer
References in periodicals archive ?
Generating safer and more efficient cells using more appropriate culture media and feeder layers provides more adequate cells for cell transplantation therapies.
Vigorously pipetted up and down to obtain single cells from plucked hair, and then centrifuged at 200xg for 5 them for specific period of time in serum based medium without feeder layer.
We now know that standardization of the method with human originated feeder layer cells and use of animal-free medium are necessary to use these cells in therapeutic applications.
After 4 passages, G418-resistant ESCs were obtained and grown on a feeder layer of primary mouse embryonic fibroblasts.
The current practice employed for the expansion of the corneal limbal stem cells is use of AM with or without the feeder layer.
We observed that, there were no apparent differences in the colony formation and growth characteristics of pigSSC colonies cultured in the presence or absence of STO cell feeder layer.
All porcine ES-like cells were derived and cultured with inactivated murine embryonic fibroblasts (MEFs) as feeder cells, and the feeder layer is one of the most important factors affecting the derivation and culture of ES cells (Li et al.
For isolation of PESCs-like, the rates of porcine blastocysts attached to feeder layers and ICM colony formation in Method B (nude embryo culture) were better than those in Method A (intact embryo culture).
GJ translation: During our procedure we did not use any mouse or human feeder layers that could possibly contain incorrect differentiation or foreign cells.
Briefly, ES cells were cultured in ES cell medium without the feeder layers for 24-28 h, then the clumps were mechanically separated and cultured in suspension in LIF-free medium containing 15% FBS for 5-10 days until EBs formed.
In addition, the cells can be efficiently cultured in tissue culture plates without the use of feeder layers (xenofree).
in 2003, but the challenge to date has been to establish appropriate derivation and growing conditions for the cells without the presence of any animal products, such as porcine enzymes, bovine serum or mouse feeder layers.