Generating safer and more efficient cells using more appropriate culture media and feeder layers
provides more adequate cells for cell transplantation therapies.
Vigorously pipetted up and down to obtain single cells from plucked hair, and then centrifuged at 200xg for 5 them for specific period of time in serum based medium without feeder layer
We now know that standardization of the method with human originated feeder layer
cells and use of animal-free medium are necessary to use these cells in therapeutic applications.
After 4 passages, G418-resistant ESCs were obtained and grown on a feeder layer
of primary mouse embryonic fibroblasts.
The current practice employed for the expansion of the corneal limbal stem cells is use of AM with or without the feeder layer
We observed that, there were no apparent differences in the colony formation and growth characteristics of pigSSC colonies cultured in the presence or absence of STO cell feeder layer
All porcine ES-like cells were derived and cultured with inactivated murine embryonic fibroblasts (MEFs) as feeder cells, and the feeder layer
is one of the most important factors affecting the derivation and culture of ES cells (Li et al.
For isolation of PESCs-like, the rates of porcine blastocysts attached to feeder layers
and ICM colony formation in Method B (nude embryo culture) were better than those in Method A (intact embryo culture).
GJ translation: During our procedure we did not use any mouse or human feeder layers
that could possibly contain incorrect differentiation or foreign cells.
Briefly, ES cells were cultured in ES cell medium without the feeder layers
for 24-28 h, then the clumps were mechanically separated and cultured in suspension in LIF-free medium containing 15% FBS for 5-10 days until EBs formed.
In addition, the cells can be efficiently cultured in tissue culture plates without the use of feeder layers
in 2003, but the challenge to date has been to establish appropriate derivation and growing conditions for the cells without the presence of any animal products, such as porcine enzymes, bovine serum or mouse feeder layers