fasting specimen

fasting specimen

Lab medicine A blood specimen drawn from a Pt who has not eaten for 12 hrs; fasting is an absolute requirement for a limited number of tests–eg, GTT; prolonged fasting causes a marked–240% ↑ in bilirubin, ↑ plasma TGs, glycerol and free fatty acids, and a marked–± 50% ↓ in glucose. See Glucose tolerance test.
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In contrast, the 2013 guidelines released by the American College of Cardiology/American Heart Association (ACC/AHA) preferred a fasting specimen for lipid testing.
Other approaches for ASCVD risk assessment include analytes such as non-HDL-C and apolipoproteins, which appear less affected by recent food intake and therefore may not require a fasting specimen.
Nonfasting sample for the determination of routine lipid profile: is it an idea whose time has come?
12) One barrier to screening is that both physicians and patients often believe that a fasting specimen is required.
Skip this step when checking lipid levels although most guidelines recommend that patients fast before lipid testing, this study found no difference between fasting and nonfasting testing for predicting mortality
A basal-state lipid profile fasting specimen determined by fasting overnight for nine to 12 hours is recommended for collection of a specimen for measurement of the lipid profile of total cholesterol, triglyceride, and HDL cholesterol, mainly because of the highly variable triglyceride diurnal variation.
Fasting lipid specimen collection
Because nonfasting specimens often contain traces of chylomicrons, calculation requires a fasting specimen (ideally >12 h).
Methods for measurement of LDL-cholesterol: a critical assessment of direct measurement by homogeneous assays versus calculation
We collect the specimen early in the day to get as close to a fasting specimen as possible.
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Fasting specimens must be collected after a 10- to 12-hour complete dietary restriction of everything except water and medications.
Quality collection: the phlebotomist's role in pre-analytical errors
Triglyceride values are also used to calculate LDL cholesterol (LDL-C) by difference using the Friedewald equation, which was derived using fasting specimens.
Fasting versus nonfasting triglycerides: implications for laboratory measurements
Obtaining SPLs only on fasting specimens can help improve test accuracy.
What is the recommended evaluation and treatment for elevated serum prolactin?
Our study was designed to determine the magnitude of changes induced by deviating from the recommended and standardized procedures of collecting fasting specimens early in the morning.
When to collect blood specimens: midmorning vs fasting samples
The Friedewald formula provides an adequate estimate of LDL-cholesterol for most fasting specimens but is known to be less reliable as triglyceride concentration increases [5].
Use of the Friedewald formula to estimate LDL-cholesterol in patients with chronic renal failure on dialysis
With plasma, only fasting specimens should be used for assessing AA status.
Ascorbic acid determination with an automated enzymatic procedure

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