fast green FCF


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fast green FCF

(fast grēn), [C.I. 42053]
An acid arylmethane dye widely used in histology and cytology and less subject to fading than light green FCF that it has replaced in many procedures; used as a quantitative cytochemical stain for histones at alkaline pH after acid extraction of DNA, and also in electrophoresis as a protein stain.
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Reaction time required for complete color development of ion-pair complexes formed between the studied drugs and fast green FCF was studied.
Sankar, "Extractive spectrophotometric determination of some anthelmintics using fast green FCF or orange-II," Indian Journal of Pharmaceutical Sciences, vol.
Qiu-hua, "Kinetic spectrophotometric determination of trace ruthenium with fast green FCF," Chinese Journal of Analysis Laboratory, vol.
Fast green FCF (Figure 2(a)) is an acidic dye used for spectrophotometric determination of some anthelmintics [32], mebeverine [33], and ceftiofur [34].
PGZ-HCl (Unipharma, El-Obour city, Egypt) working solution 50 [micro]g[mL.sup.-1] and for molar ratio 1 x [10.sup.-4] M solution were prepared by dissolving in the least amount of 0.1 M HCl and then completing to volume with distilled water for fast green FCF method, while for orange G method, working solution 1000 [micro]g[mL.sup.-1] and, for molar ratio, 2 x [10.sup.-3] M solution were prepared by dissolving in the least amount of 0.5 M [H.sub.2][SO.sub.4] and then completing to volume with distilled water.
CRV (Amoun Pharmaceutical Co, El-Obour city, Egypt) working solution 50 [micro]g[mL.sup.-1] and for molar ratio 1 x [10.sup.-4] M solution were prepared by dissolving in the least amount of methanol and then completing to volume with distilled water for fast green FCF method, while for orange G method, working solution 500 [micro]g[mL.sup.-1] and for molar ratio, 7 x [10.sup.-4] M solution were prepared by dissolving in the least amount of methanol and then completing to volume with distilled water.
Fast green FCF reacts with PGZ-HCl and CRV to yield a blue colored complex exhibiting maximum absorption at 631 nm (Figures 10 and 11), and the ion pair formed can be extracted with methylene chloride.
The Codex Committee on Food Additives has adopted nine colors for use in food supplements at levels consistent with recommendations by the International Alliance of Dietary/Food Supplement Associations (IADSA).The committee agreed to move allura red AC (300 mg/kg); caramel color, class IV (20,000 mg/kg); carotenoids (300 mg/kg); chlorophylls, copper complexes (500 mg/kg); erythrosine (300 mg/kg); fast green FCF (600 mg/kg); grape skin extracts (500 mg/kg); in-digotine (300 mg/kg); and iron oxides (7500 mg/kg) forward for the Codex General Standard for Food Additives (GSFA), which sets conditions for permitted food additives to be used in all foods.
3 Sea green Beverages, puddings, ice cream Fast Green FCF sherbet, cherries, confection, baked goods, dairy products FD&C Red No.

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