cDNA sequences for transcription factors and signaling proteins of the hemichordate Saccoglossus kowalevskii: efficacy of the expressed sequence tag
(EST) approach for evolutionary and developmental studies of a new organism.
Thus, for example, when Merck decided to sponsor the Merck Genome Initiative to generate partial gene sequences (expressed sequence tags
or ESTs)(27) in the public domain, two other private firms already had a significant lead over the Merck-sponsored effort in generating private databases of ESTs.(28) It is plausible that by putting ESTs in the public domain, Merck hoped to create prior art that would defeat future patent claims to the full-length genes corresponding to the ESTs, although it now seems unlikely that such a strategy will prove successful.(29)
The precursor to this approach would be sequencing Expressed Sequence Tags
using Sanger sequencing technology.
Gocayne et al., "Complementary DNA sequencing: expressed sequence tags
and human genome project," Science, vol.
Abbreviations: EST, expressed sequence tag
; UTR, untranslated region.
The company's LifeSeq Gold database integrates expressed sequence tag
(EST) and full-length gene sequence information, mapping data and public genomic sequence information.
Expressed sequence tag
(EST) sequencing has been considered an efficient approach for genomic study and functional gene identification, especially for those species without a genome reference.
To amplify the ORF region of the PtCacyBP gene, this study used the previously obtained PtCacyBP expressed sequence tag
sequence (PT0030E11) and designed two primers: PtCacyBP-F1 (5' CGC TCG AGG ATG TCT AAC AAG CTG GAG GAG 3', position: 1-21, see Fig.
The array elements were selected from 6,494 cDNA clones identified in a large-scale expressed sequence tag
The main databases serving as the targets for MS data searches are the expressed sequence tag
and the protein sequence databases, which contain protein sequence information translated from DNA sequence data (29).
The NCBI database and an expressed sequence tag
database (Mytibase) of transcribed sequences of Mytilus galloprovincialis (Venier et al.
An expressed sequence tag
database of T-cell-enriched activated chicken splenocytes: sequence analysis of 5251 clones.