We report a protocol that describes the isolation and culture of human dental pulp mesenchymal stem cells from permanent and deciduous teeth using explant
Similarly, the highest mean number of bulblets per explant
(3.13) was found on Lindeman Orchid medium containing 8 mg/l BAP.
Conversely, CHEN and CHANG (2006) have reported that the embryos' permanence in an inducing nutritional medium containing TDZ led to the formation of secondary embryos and; consequently, to the increased number of embryos per explant
. The conversion of somatic embryos into plants is the last somatic embryogenesis stage and it happens in nutrient medium lacking (or not) growth regulators (VAN DER VYVER, 2010; BASNAYAKE et al., 2011).
The inocultion was performed by immersing the explants
in the bacterial suspension for 15 min under constant agitation (900 rpm), and bacterial suspension excess was eliminated with sterile towel paper.
Figure 3A shows explant
and shoot growth (cm), where explant
length was higher under immersion conditions of 5x/day in liquid MS culture medium.
disinfection for testing was so that plants were placed under running water for primary disinfection for 30 minutes after primary washing with tap water.
showed stellate portal fibrosis with vascular proliferation, typical of Symmers' fibrosis and in four cases there were schistosomal granulomas (Fig.
The experimental design was a randomized complete block design, with factorial 2X3X2 (auxin x cytokinin x explant
type), with 8 blocks and 2 replicates each.
For Pinus taeda, a nine-day induction period in medium with regulators (2.68 uM NAA and 0.44 uM BA), and subsequent subculture onto medium containing only water/agar without sucrose, favoured rooting in 35% of explants
after six weeks of cultivation with an average of 2.3 roots per explant
(CEZAR et al., 2015).
Shoot tip and nodal explants
of Salvadora persica and Solanum suratensse were used as an explants
, collected from the experimental field of Plant Tissue Culture Laboratory, PCSIR Labs Complex Karachi.
It suggests that the genetic transformation system developed using cotyledonary node explants
is efficient and successful in cucumber.
A protocol using droplet vitrification technique (Panis, Piette, & Swennen, 2005) was successfully performed using shoot tips as explants
(Santos et al., 2015).