explant


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explant

 
1. (eks-plant´) to take from the body and place in an artificial medium for growth.
2. (eks´plant) tissue taken from the body and grown in an artificial medium.

ex·plant

(eks-plant'),
Living tissue transferred from an organism to an artificial medium for culture.

explant

(ĕk-splănt′)
tr.v. ex·planted, ex·planting, ex·plants
To remove (living tissue) from the natural site of growth and place in a medium for culture.
n. (ĕks′plănt′)
Explanted tissue.

ex′plan·ta′tion n.

explant

Transplantation medicine An organ that requires removal due to rejection, after transplantation. See Transplantation.

ex·plant

(eks-plant')
Living tissue transferred from an organism to an artificial medium for culture.

explant

any actively dividing plant tissue that can be induced to produce CALLUS tissue in tissue culture.
References in periodicals archive ?
We report a protocol that describes the isolation and culture of human dental pulp mesenchymal stem cells from permanent and deciduous teeth using explant method.
Similarly, the highest mean number of bulblets per explant (3.13) was found on Lindeman Orchid medium containing 8 mg/l BAP.
Conversely, CHEN and CHANG (2006) have reported that the embryos' permanence in an inducing nutritional medium containing TDZ led to the formation of secondary embryos and; consequently, to the increased number of embryos per explant. The conversion of somatic embryos into plants is the last somatic embryogenesis stage and it happens in nutrient medium lacking (or not) growth regulators (VAN DER VYVER, 2010; BASNAYAKE et al., 2011).
The inocultion was performed by immersing the explants in the bacterial suspension for 15 min under constant agitation (900 rpm), and bacterial suspension excess was eliminated with sterile towel paper.
Figure 3A shows explant and shoot growth (cm), where explant length was higher under immersion conditions of 5x/day in liquid MS culture medium.
Explants disinfection for testing was so that plants were placed under running water for primary disinfection for 30 minutes after primary washing with tap water.
All explants showed stellate portal fibrosis with vascular proliferation, typical of Symmers' fibrosis and in four cases there were schistosomal granulomas (Fig.
The experimental design was a randomized complete block design, with factorial 2X3X2 (auxin x cytokinin x explant type), with 8 blocks and 2 replicates each.
For Pinus taeda, a nine-day induction period in medium with regulators (2.68 uM NAA and 0.44 uM BA), and subsequent subculture onto medium containing only water/agar without sucrose, favoured rooting in 35% of explants after six weeks of cultivation with an average of 2.3 roots per explant (CEZAR et al., 2015).
Shoot tip and nodal explants of Salvadora persica and Solanum suratensse were used as an explants, collected from the experimental field of Plant Tissue Culture Laboratory, PCSIR Labs Complex Karachi.
It suggests that the genetic transformation system developed using cotyledonary node explants is efficient and successful in cucumber.
A protocol using droplet vitrification technique (Panis, Piette, & Swennen, 2005) was successfully performed using shoot tips as explants (Santos et al., 2015).