exonuclease


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exonuclease

 [ek″so-noo´kle-ās]
an enzyme that cleaves single mononucleotides from the end of a polynucleotide chain.

ex·o·nu·cle·ase

(ek'sō-nū'klē-ās),
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid); several have been prepared from Escherichia coli, designated exonuclease I, exonuclease II, etc.; exonuclease III, which removes nucleotides from 3' ends of DNA, is used in DNA sequencing. Compare: endonuclease.

exonuclease

(ĕk′sō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
n.
Any of a group of enzymes that catalyze the hydrolysis of single nucleotides from the end of a DNA or RNA chain.

ex·o·nu·cle·ase

(eks'ō-nū'klē-ās)
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid).
Compare: endonuclease

exonuclease

an enzyme that removes a terminal NUCLEOTIDE (3' or 5') in a POLYNUCLEOTIDE CHAIN. Exonucleases remove the nucleotides in a successive way, one by one, and are highly specific in their action.

ex·o·nu·cle·ase

(eks'ō-nū'klē-ās)
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid).
Compare: endonuclease
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References in periodicals archive ?
Tentacle Probe reaction mechanism with exonuclease active polymerase is shown in Fig.
Tentacle probes[TM]: differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time PCR
krusei (42.5 mg/L) were digested with 10 units of lambda exonuclease (New England Biolabs) at 37 [degrees]C in the PCR buffer described above for 5 min unless otherwise noted.
Rapid exonuclease digestion of PCR-amplified targets for improved microarray hybridization
For your SCA3 assay, do you use a thermostable polymerase that has 3' to 5' exonuclease activity?
The Power of Proficiency Testing: Unraveling Single-Nucleotide Polymorphism Interference, With Potential Impact on Clinical Testing of Spinocerebellar Ataxia Type 3
Cheng, Novel interaction of aphidicolin with herpes simplex virus DNA polymerase and polymerase-associated exonuclease, J.
Inhibitory Mechanism Exhibited by Phenol-based Natural Products against DNA Polymerase [alpha] from Psoralea corylifolia by Molecular Docking
Both DNA and RNA are generally linear tetrapolymers, and nuclease enzymes known as exonucleases hydrolytically cleave these types of polymers at their ends (e.g., Phosphodiesterase I), while endonuclease enzymes cleave the polymer chain at internal locations (e.g., DNase I, EcoRI).
Self-Sterilizing/DNA-Free Coatings In Space and On Earth
Gregory, "A role for the Perlman syndrome exonuclease Dis312 in the Lin28-let-7 pathway," Nature, vol.
The Molecular Basis and Therapeutic Potential of Let-7 MicroRNAs against Colorectal Cancer
Three prime repair exonuclease 1 (TREX1) gene mutation, which encodes a potent DNA exonuclease, generates dysfunctional DNA degradation and may result in the accumulation of single- or double-stranded DNA degradation products that could trigger an autoimmune response (76-78).
DNA Damage and Deficiencies in the Mechanisms of Its Repair: Implications in the Pathogenesis of Systemic Lupus Erythematosus
Five ul PCR products were mixed with 1 ul exonuclease and 1ul alkaline phosphatase for purification.
Type and Frequency of mutations in katG and rpoB genes in Multi-Drug Resistant Strains of Mycobacterium Tuberculosis Complex
Nevertheless, the GDS04 strain shares 95.7% aa identity with HKU2 in nonstructural protein (nsp) 3 (adenosine diphosphate-ribose 1'-phosphatase), 96.4% in nsp5 (3C-like protease), 94.6% in nspl2 (RNA-dependent RNA polymerase), 99.8% in nspl3 (helicase), 99.2% in nspl4 (3'-to-5' exonuclease), 99.4% in nspl5 (poly[U]-specific endoribonuclease), and 97.6% in nspl6 (2'-O-ribose methyltransferase).
A new bat-HKU2-like Coronavirus in swine, China, 2017
The company said chemically modified RNA improves in vivo stability by protecting RNA molecules against exonuclease attack, allowing it to persist longer in certain cell Types and regions of genomic DNA that can be challenging to edit using conventional guide RNAs.
Modified synthetic sgRNA for editing primary, stem cells
Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities.
The genetic enablement of the blue crab Callinectes sapidus
PCR from 20 clones that exhibited a single amplified band of 500-1000 base pairs were cleaned with 16 units of Exonuclease I and 3 units of Antarctic Phosphatase (New England Biolabs) followed by ethanol precipitation.
Characterization and isolation of ten microsatellite loci in false map turtles, graptemys pseudogeographica (Emydidae, Testudines)

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