exonuclease


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exonuclease

 [ek″so-noo´kle-ās]
an enzyme that cleaves single mononucleotides from the end of a polynucleotide chain.

ex·o·nu·cle·ase

(ek'sō-nū'klē-ās),
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid); several have been prepared from Escherichia coli, designated exonuclease I, exonuclease II, etc.; exonuclease III, which removes nucleotides from 3' ends of DNA, is used in DNA sequencing. Compare: endonuclease.

exonuclease

(ĕk′sō-no͞o′klē-ās′, -āz′, -nyo͞o′-)
n.
Any of a group of enzymes that catalyze the hydrolysis of single nucleotides from the end of a DNA or RNA chain.

ex·o·nu·cle·ase

(eks'ō-nū'klē-ās)
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid).
Compare: endonuclease

exonuclease

an enzyme that removes a terminal NUCLEOTIDE (3' or 5') in a POLYNUCLEOTIDE CHAIN. Exonucleases remove the nucleotides in a successive way, one by one, and are highly specific in their action.

ex·o·nu·cle·ase

(eks'ō-nū'klē-ās)
A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid).
Compare: endonuclease
References in periodicals archive ?
krusei (42.5 mg/L) were digested with 10 units of lambda exonuclease (New England Biolabs) at 37 [degrees]C in the PCR buffer described above for 5 min unless otherwise noted.
Cheng, Novel interaction of aphidicolin with herpes simplex virus DNA polymerase and polymerase-associated exonuclease, J.
Both DNA and RNA are generally linear tetrapolymers, and nuclease enzymes known as exonucleases hydrolytically cleave these types of polymers at their ends (e.g., Phosphodiesterase I), while endonuclease enzymes cleave the polymer chain at internal locations (e.g., DNase I, EcoRI).
Gregory, "A role for the Perlman syndrome exonuclease Dis312 in the Lin28-let-7 pathway," Nature, vol.
Three prime repair exonuclease 1 (TREX1) gene mutation, which encodes a potent DNA exonuclease, generates dysfunctional DNA degradation and may result in the accumulation of single- or double-stranded DNA degradation products that could trigger an autoimmune response (76-78).
Five ul PCR products were mixed with 1 ul exonuclease and 1ul alkaline phosphatase for purification.
Nevertheless, the GDS04 strain shares 95.7% aa identity with HKU2 in nonstructural protein (nsp) 3 (adenosine diphosphate-ribose 1'-phosphatase), 96.4% in nsp5 (3C-like protease), 94.6% in nspl2 (RNA-dependent RNA polymerase), 99.8% in nspl3 (helicase), 99.2% in nspl4 (3'-to-5' exonuclease), 99.4% in nspl5 (poly[U]-specific endoribonuclease), and 97.6% in nspl6 (2'-O-ribose methyltransferase).
The company said chemically modified RNA improves in vivo stability by protecting RNA molecules against exonuclease attack, allowing it to persist longer in certain cell Types and regions of genomic DNA that can be challenging to edit using conventional guide RNAs.
Trypanosome U-deletional RNA editing involves guide RNA-directed endonuclease cleavage, terminal U exonuclease, and RNA ligase activities.
PCR from 20 clones that exhibited a single amplified band of 500-1000 base pairs were cleaned with 16 units of Exonuclease I and 3 units of Antarctic Phosphatase (New England Biolabs) followed by ethanol precipitation.