Viability of Cryptosporidiumparvum oocysts: correlation of in vitro
excystation with inclusion or exclusion of fluorogenic vital dyes.
Excystation occurs in the small intestine with the release of motile trophozoites, which migrate to the large intestine.
Followed by oocytes swallowing, these are exposed to enzymatic actions in intestine and grinding in gizzard thus leading to liberation of sporozoites termed as
excystation. Then sporozytes penetrate epithelial tissues of small intestine, transported by macrophages to depth of intestinal glands where advanced development start over (Jeurissen and Veldman, 2002).
In the small intestine occurs the
excystation and the trophozoites emerge.
Smith, "The effect of blueberry extracts on Giardia duodenalis viability and spontaneous
excystation of Cryptosporidium parvum oocysts, in vitro," Methods, vol.
In addition, some [alpha]-giardins also participate in the encystation and
excystation process of Giardia cysts, preventing their leakage of the cell membrane and disintegration of the cellular structure under the environment of high intestinal bile salts [6].
We found water temperatures up to 34[degrees]C, which may facilitate
excystation of N.
Sarcocystis giantea: studies on sporocyt production,
excystation and viability.
It is transmitted through oral-fecal route.31 After ingestion, the giardia cysts undergo
excystation and attach to the enterocytes and stimulate the process of apoptosis.
Excystation then occurs in the terminal ileum or colon, resulting in trophozoites, which is an invasive form of the parasite.
Following ingestion,
excystation takes place shortly after cysts leave the stomach and leads to rapid colonisation of the duodenum and jejunum, where the excysted trophozoites attach to the intestinal mucosa and multiply rapidly.