Euploid embryos were identified via 24-chromosome PGS using either an array-based comparative genomic hybridization or an array-based single nucleotide-polymorphism method, as previously described.[23] Selected embryos were vitrified, warmed, and transplanted as previously described.[24]
Costa and Grybko [14] observed a small but significant reduction on the levels of CA1 LTP induced by TBS in hippocampal slices from Ts65Dn mice compared with
euploid control mice.
PGD is advised in cases where a specific aberration is isolated (single-gene disorders, inherited genetic diseases, and X-linked mutations), while PGS is carried out in infertile patients for the purpose of selecting the most viable
euploid embryo [16].
The number of
euploid embryos identified by PGS from each of the 724 cycles analysed was then correlated with the number of oocytes retrieved in that cycle.
The recommendations state that
euploid embryo transfer should be limited to one for women of any age with a favorable prognosis, and for women younger than 35 years regardless of the embryo stage.
The patient underwent 2 stimulation cycles in a GnRH-antagonist protocol; however there was no
euploid embryo after PGS, so no embryo transfer was done.
Notwithstanding, this does not mean that an
euploid embryo will necessarily implant and develop as some other reasons such as inappropriate endometrial receptivity might also cause implantation failure even in normal embryos [47-50].
Accordingly, in Results, the text reading "The number of
euploid embryos was negatively related to the OSI (p = 0.04), which indicates that the women who produced more oocytes in response to lower gonadotropin doses (low OSI) were those who displayed more
euploid" should be changed to "The number of
euploid embryos was positively related to the OSI (p = 0.04), which indicates that the women who produced more oocytes in response to lower gonadotropin doses (high OSI) were those who displayed more
euploid."
Blastocysts needed to transfer at least one
euploid embryo: data from 10,852 pre-implantation genetic screening (PGS) cycles, Fertility and Sterility.
In a case diagnosed as complete trisomy 9 by conventional cytogenetic techniques, fluorescence in situ hybridization (FISH) studies of metaphase and interphase blood cells and skin fibroblasts could identify the presence of
euploid and trisomy 9 cells.
Of the 10 embryos subjected to array CGH, 2 failed to amplify, 2 were
euploid, 4 were aneuploid and 2 had unbalanced translocations.
GSE 1281 included six Down syndrome samples and six normal samples, which were from newborn rat brain tissue; GSE 1611 contained 12 samples (Ts1Cje and
euploid Down syndrome cerebellum) as follows: two Down syndrome samples at postnatal days 15 and 30, respectively, and two normal samples of newborn at postnatal days 15 and 30, respectively.
