The genitalia and wing venation were examined in 60% euparal
solution or dried condition, respectively.
We dehydrated specimens in 95% ethanol before dissecting and slide mounting all specimens in Euparal
. Specimens were either slide-mounted individually or in multiples.
Slide mounting medium appears to be Euparal
and remains clear.
Las alas eran previamente montadas en euparal
Los ejemplares fueron macerados con hidroxido de potasio al 8% en bano Maria, lavados en agua destilada caliente, acido acetico al 10%, alcohol isopropilico mezclado con aceite de clavo, aceite de clavo puro y finalmente incluidos en resina Euparal
. Los terminos morfologicos y de nomenclatura empleados fueron basados en Amorim & Rindal (2007) y Jaschhof (2005).
The Chironomidae larvae were fixed on slides with Euparal
, separating the head capsule from the rest of the body.
Wings of illustrated extant specimens were cut off specimens preserved dry or under ethanol, softened as necessary, and mounted in white Euparal
(Asco Laboratories, Manchester, UK).
On the mount, genitalia are put in a small drop of Euparal
after being rinsed in water and soaked in 100 % ethanol.
Slides were made permanent by freezing with CO2, removing the cover slip, dehydrating in absolute alcohol and mounting in Euparal
Preparations were mounted in Euparal
on slides following Robinson (1976) and viewed at 100400x magnification.
The organs were mounted in euparal
on slides and examined under optical microscope.
Oxidacion de la muestra con HNO3 y montaje de las laminas usando hydroxylethyl celulosa (HEC) para homogenizar las particulas organicas en el cubreobjetos y euparal
o balsamo de Canada para fijarlo permanentemente sobre el portaobjetos.