A medium for mounting histologic specimens, composed of sandarac, eucalyptol, paraldehyde, camphor, and phenyl salicylate.
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The genitalia and wing venation were examined in 60% euparal solution or dried condition, respectively.
We dehydrated specimens in 95% ethanol before dissecting and slide mounting all specimens in Euparal. Specimens were either slide-mounted individually or in multiples.
Los ejemplares fueron macerados con hidroxido de potasio al 8% en bano Maria, lavados en agua destilada caliente, acido acetico al 10%, alcohol isopropilico mezclado con aceite de clavo, aceite de clavo puro y finalmente incluidos en resina Euparal. Los terminos morfologicos y de nomenclatura empleados fueron basados en Amorim & Rindal (2007) y Jaschhof (2005).
Wings of illustrated extant specimens were cut off specimens preserved dry or under ethanol, softened as necessary, and mounted in white Euparal (Asco Laboratories, Manchester, UK).
On the mount, genitalia are put in a small drop of Euparal after being rinsed in water and soaked in 100 % ethanol.
Slides were made permanent by freezing with CO2, removing the cover slip, dehydrating in absolute alcohol and mounting in Euparal.
The organs were mounted in euparal on slides and examined under optical microscope.