The inactivation pathway occurs mainly in the liver via conjugation into testosterone conjugates (glucuronides and to a lesser extent sulfates) and in equal proportions into the 17-ketosteroids (androsterone and etiocholanolone
USP revealed an increased etiocholanolone
of 1.31 mg/day (4.5 [micro]mol/day) [RI, <0.35 (<1.2)] and a low androsterone of 0.09 mg/day (0.3 [micro]mol/day) [RI, <0.44 (<1.5)], resulting in extremely low 5[alpha]- to 5[beta]-reduced steroid metabolites ratio of 0.06 (RI, 0.5-1.9).
A 24-hour-urine adrenal steroid profile by GC-mass spectrometry showed robust Cortisol and cortisone but low precursors and, significantly, excessive 5[alpha]-R activity by increased androsterone relative to etiocholanolone
. On four-hour oral glucose tolerance test with insulin, her fasting values of glucose (90 mg/dL) and insulin (3.2[micro]U) were normal, but her 30-minute insulin response was sluggish; the two-hour glucose was excessive at 151, and she "felt like passing out" at the fourth hour, with glucose only 59 - a weak counter-regulatory response.
Continued from page 1 possibility of tampering as the system is foolproof." A random dope test was conducted on the athlete in the last week of June and Inderjeet's ' A' sample tested positive for steroids androsterone and etiocholanolone
In the liver, testosterone is converted by a series of enzymatic steps into androsterone, etiocholanolone
, DHT, and 3-androstanediol.
CMap name Enrichment P 8-Azaguanine 0.932 0.00004 Apigenin 0.86 0.00052 Chrysin 0.931 0.00056 Sulfametoxydiazine 0.855 0.00056 Lycorine 0.803 0.00068 Digoxin 0.846 0.0008 Prochlorperazine 0.467 0.00086 Sanguinarine 0.977 0.00087 Helveticoside 0.733 0.00097 Felbinac -0.847 0.00101 Adiphenine -0.771 0.00118 Diloxanide -0.83 0.00157 Etiocholanolone
-0.704 0.00157 Heptaminol -0.753 0.0017 Acetylsalicylic acid 0.499 0.00174 Proscillaridin 0.903 0.00182 Cinchonine -0.82 0.00197 0316684-0000 -0.813 0.00229 Proadifen 0.806 0.00265 DL-Thiorphan 0.956 0.00342
Beta nicotinamide adenine dinucleotide ([beta]-[NAD.sup.+]), etiocholanolone
and dimethyl sulfoxide were from Sigma Chemical Co.
The research has revealed that in certain rare circumstances, a chemical reaction can occur in the bottle after a urine sample has been provided, whereby two endogenous hormones, androsterone (A) and etiocholanolone
(E), are converted into 19-NA and 19-NE, metabolites of nandrolone that are tested for in doping analysis.
% of Median Androgens 24hr Dry DHEA 13% 18% Androsterone 37% 48% Etiocholanolone
57% 69% Testosterone 21% 18% 5a-Ansdrostanediol 55% 60% 5b-Ansdrostanediol 27% 43% Glucocorticoids and Mineralocorticoids
The testosterone metabolites androsterone and etiocholanolone
can be measured in 24-hour urine, while the best androgen metabolites in the serum to assess are dihydrotestosterone and androstanediol glucuronide, which concentration is on the average double that of testosterone.
Simultaneous OF testosterone, epitestosterone, dihydrotestosterone, DHEA, androsterone, etiocholanolone
, and [alpha]- and [beta]-androstanediol quantification collected with the Quantisal device were presented, but never published (40).
A second laboratory metric is the balance of 5alpha- to 5beta-reduced steroid metabolites (e.g., androsterone to etiocholanolone
) in GC/mass-spec studies of 24-hour urine.