Cysteine, ascorbic acid, sodium sulfite, citric acid, thiourea, sodium azide, ethylenediamine tetraacetic acid
disodium (EDTA) and sodium diethyldithiocarbamate (DETC) were used as PO inhibitors.
It has been shown that high biomass-producing plant species such as Indian mustard (Brassica juncea), corn (Zea mays), and sunflower (Helianthus annus) can accumulate significant amounts of metals when the solubility of these metals is induced through the addition of chemicals such as EDTA (ethylenediamine tetraacetic acid
) for Pb or EGTA (ethylene glycol tetraacetic acid) for Cd (Garbisu and Alkorta 2001).
EDTA (ethylenediamine tetraacetic acid
) chelates the metals-that is, it traps impurities that would otherwise make oils rancid and break down artificial colors.
Three separate samples of blood (10 mL each) were drawn into ethylenediamine tetraacetic acid
The powder was then ground to a paste with ice-cold buffer [0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.5, 20% (v/v) glycerol, 1% (w/v) polyvinylpolypyrrolidone, 1% (v/v) Triton X, 5 mM ethylenediamine tetraacetic acid
(EDTA), 1 mM dithiothreitol (DTT), and 1 mM phenylmethylsulfonyl fluoride] at a ratio of 4 mL [g.sup.-1] fresh wt.
The now-preferred ethylenediamine tetraacetic acid
(EDTA) anticoagulant (1.5 mg of EDTA/mL of blood) results in minimal problems in the usual CBC components, including the reticulocyte count.
Each worker received three 5-day chelation treatments with intravenous calcium disodium ethylenediamine tetraacetic acid
approximately 15 days apart.
Macromolecules were precipitated by mixing equal volumes of liver homogenate (20 percent, w/v) and trichloroacetic acid (10 percent, w/v) containing 5 mM ethylenediamine tetraacetic acid
(EDTA), and centrifuged at 5000g for 15 min.
Protein extracts were prepared from the human tissues and cells using a lysis buffer supplemented with ethylenediamine tetraacetic acid
(EDTA)-free complete protease inhibitors (Roche, Mannheim, Germany).