EBN group: Normal Diet and
ethidium bromide (EB) injection.
Results were recorded after electrophoresis on 2% agarose gel and staining with
ethidium bromide.
Even when the procedure is optimized for DNA in agarose, silver staining of DNA will only give sensitivity similar to
ethidium bromide18.
Agarose 2%, and TBE (1X) at (75 V/cm for 90 min., stained with
Ethidium bromide dye and visualized on a UV transilluminator.
Following PCR, aliquots (20 [micro]L) of the reaction mixtures were analysed by electrophoresis on a 1.5% Agarose gel containing
ethidium bromide (0.2 mg/mL), in the presence of an appropriate DNA molecular weight marker.
For the indirect detection,
ethidium bromide solution (15 [micro]L, 5.0 x [10.sup.-6] mol [L.sup.-1]) was used as indicator.
Although different tests are available, in the present study,
ethidium bromide/acridine orange viability staining and micronucleus assay were used.
In this regard, different physical mutagens including X-rays, -rays, ultraviolet (UV) rays or chemical mutagens such as N-methyl-N'- nitro-N-nitrosoguanidine (MNNG), ethyl methanesulfonate (EMS) and
ethidium bromide (EtBr) have been reported (Abdelghani et al., 2005; Iftikhar et al., 2010).
Electrophoresis was operated for three hours at 120 volts,
ethidium bromide was used as detecting dye and gel was placed in (0.5ug/ml) solution of
ethidium bromide.
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After 7 h, RPE cell death was monitored by propidium iodide (PI) using FACS analysis and by
ethidium bromide and acridine orange fluorescent staining.
Ethidium bromide (EtBr) is an intercalating molecule that binds by intercalation between the base pairs of DNA strands and is extremely useful tool in different techniques (Palchaudhuri and Hergenrother, 2007).