The established cell line
, Hela cells, was found to have same effect on chicken spermatozoa motility and fertility as epithelial cells from fowl shell gland (Ashizawa et al., 1982).
Most of the studies in this issue applied in vitro (using established cell line
) and in vivo (animal models and human patients) tests to evaluate the efficacy of anticancer medicinal plants and phytochemicals activity and their action of mechanism.
After staining with Hoechst 33258, fibroblast nuclei appeared as blue ellipses, showing that the established cell line
was mycoplasma negative (Fig.
Building on previous work from Cambridge which showed that it was possible to transform skin cells into liver cells by reprogramming stem cells, the team successfully and accurately corrected an alpha 1-antitrypsin gene in an established cell line
containing the mutation.
In the final step of the project, they took cells directly from a patient with alpha1-antitrypsin deficiency and corrected the mutation exactly as they had with the established cell line
. The corrected cells produced normal alpha1-antitrypsin protein.
The difference between the approach of the AG and that of the Enlarged Board in WARF, is that the latter still left the door open to the Convention States to determine (a) what was a human embryo, and (b) what 'use' necessarily results in the destruction of such an embryo (for example, could this include the use of HeS from an already established cell line
?); which in turn left the door open to patents for the use of pluripotent HeS.
(2) HeLa cells are an established cell line
used in medical research.
We found that the genetic results reported by the submitting laboratories were reliable and that, therefore, analysis of DNA derived from the established cell line
was sufficient to confirm the presence of the mutation.
Immunobiologic, cytogenetic and drug response features of established cell line
(SCRC-1) from renal small cell carcinoma.
For every primary established cell line
, not only molecular but also physiological parameters should be estimated to enable a more precise future clustering of different GB cases.
In the present study, we used the combined methods of cell viability, microorganism detection, chromosome analysis, isoenzyme analysis and fluorescent protein genes transfection to detect the established cell line
. The average viability of cells detected that the cells were healthy in culture conditions and that freezing had little influence on the viability of the cells.
*Emergence of novel fabrication techniques contributes to the growth of scaffold-free 3D cell culture systems *Rapid adoption of culturing systems in cancer research for development of immortalized established cell lines
or primary cell cultures to perform in vivo drug screening has led to its largest revenue share *3D bioprinting enables creation of 3D printed building blocks of embryonic stem cells, which could be further used for micro-organ generation and tissue regeneration experiments *Changing of regulatory policies for stem cell research and high potential of 3D culturing tools fuels the stem cell research segment *North America dominated the global 3D cell culture market with a share of over 41.0% in 2016.