diacetylactis produced acetoine, all possessed arginine dihydrolase and all hydrolyze
esculin and growth on citrate.
Esculin, were applied to measure the activities of AY- glucosidase.
5% isolates exhibited
esculin hydrolysis, while starch hydrolysis was shown by 70% of the strains.
In nature, horse-chestnut tree bark has an
esculin content of three percent.
1%
esculin (Soriano, Fernandez Roblas, Calvo, & Garcia Calvo, 1998), previously adjusted to the 0.
All isolates were confirmed as enterococci [grew on enterococcosel agar (BBL), were catalase negative, hydrolyzed
esculin, grew in 6% NaCl broth, and grew at 44.
Versalovic, 2011) Table 2: Phenotypic characteristics of Leptotrichia species Species Catalase
Esculin Beta- hemolysis L.
For FE confirmation, after 48 h at 35[degrees]C, filters with red colonies were placed on
Esculin Iron Agar (Difco) for 20-30 min, and those red colonies surrounded with a brown-black precipitate were counted.
dark colour caused by
esculin hydrolysis, were plated individually on BBL CHROM agar and PALCAM agar (Oxoid), and the plates were incubated at 37 [degrees]C for 48 hours.
Enterococcus fecalis was identified by its ability to hydrolyze
esculin in the presence of bile using the bile
esculin medium [14].
Iso-lates were identified to the genus and species level based on the standard biochemical and microbiologi-cal methods such as: morphologic appearance on Gram-stain (gram negative coccobacilli), oxidase negative, catalase and citrate positive, nonmotile, negative
esculin hydrolysis, growth at 41oC and nega-tive lysine decarboxylase.
Acid was produced from glucose, mannitol, inositol, sorbitol, sucrose, melibiose,
esculin, lactose, salicin, xylose, adonitol, saccharose, trehalose cellobiose, raffinose and arabinose.
