esculin


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es·cu·lin

(es'kyū-lin),
A glucoside from horse-chestnut bark; used as a sun block.
[L. aesculus, the Italian oak]
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Biochemical reaction Result Nitrate + Growth in MacConkey + Urea hydrolysis + Esculin hydrolysis + Gelatin hydrolysis - ONPG (o-nitrophenyl-[beta]-D-galactopyranoside) + Oxidase + Vogesproskauer - [beta]-Galactosidase - Tryptophan deaminase - Ornithine decarboxylase - Lysine decarboxylase - Arginine decarboxylase - Motility + Glucose (assimilation) + Mannose + Mannitol + Maltose + Gluconate + Arabinose + Adonitol + Glutamyl arylamidase pNA + Indole -
To determine the biotype of isolates, esculin, indole, and lipase activity were investigated.
The selected LAB strains were assessed for the shape, cell wall composition (Gram staining), catalase production, carbohydrate, and sugar fermentation (amygdalin, arabinose, cellobiose, esculin, fructose, galactose, glucose, lactose, maltose, mannitol, raffinose, rhamnose, ribose, sorbitol, sucrose, and trehalose) as described in the Bergey's Manual of Determinative Bacteriology.
Anaerobic culture yielded irregular, grey, non-hemolytic, molar tooth colonies of Gram-positive bacilli, negative for catalase, urease, and indole tests but positive for esculin hydrolysis test, identified as A.
Entero-set consisting of growth on esculin agar in the presence of 40% bile, 6.5% NaCl, and arabinose test was used to identify Enterococci.
Further, the bacterial isolate decarboxylated arginine but did not decarboxylate lysine and ornithine and hydrolysed gelatin but did not hydrolyse esculin. It did not produce urease, indole, and DNase.
Mean content (mg/100 ml) Molecule Alcohol- Ale free 2,3-Dihydroxy-1-guaiacylpropanone 0.0025 3-Methylcatechol 4-Ethylcatechol 4-Hydroxycoumarin 4-Methylcatechol 4-Vinylguaiacol 0.0100 4-Vinylphenol Catechol Esculin Pyrogallol Tyrosol 0.2700 Umbelliferone Vanillin 0.0048 Mean content (mg/100 ml) Molecule Dark Regular 2,3-Dihydroxy-1-guaiacylpropanone 0.0034 3-Methylcatechol 0.0029 0.0001 4-Ethylcatechol 0.0010 0.0006 4-Hydroxycoumarin 0.1100 4-Methylcatechol 0.0022 4-Vinylguaiacol 0.0300 0.1500 4-Vinylphenol 0.0300 0.0045 Catechol 0.0100 0.0011 Esculin 0.0200 Pyrogallol 0.0300 0.0047 Tyrosol 0.3200 Umbelliferone 0.0017 Vanillin 0.0200 Data from the Phenol-Explorer database [12].
Esculetin, esculin, fraxin, and fraxetin are some of the pharmacologically active components isolated from different species of Fraxinus plant (Figure 2).
iniae: acetoin production (Voges Proskauer) (Remel, USA), aminopeptidase leucine (LAP) (Remel, USA), arylamidase pyrrolidonyl (PYR) (Remel, USA), starch hydrolysis (Merck, Germany) and esculin hydrolysis (Neogen, Acumedia, USA).
Dextran production on MSE medium [22], citric acid degradation on Kempler and McKay solid medium, esculin hydrolysis and production of acetoine on Clark and Lubs medium [23,24].