Erythrosin B is approved in the United States for food and ingested drugs with an ADI of 2.
Given the ADIs and estimated environmental concentrations, low absorption rates such as 1% could still result in body burdens in the nanomolar to micromolar range for erythrosin B and tartrazine.
The adverse effects reported by others coupled with the chemical similarities among tartrazine, sudan I, erythrosin B, and estradiol-17[beta] (E2) (Fig.
Porcine insulin, EDTA, charcoal-dextran, E2 (E8875-IG), tamoxifen (T5648-1G), tartrazine (T0388-100G), erythrosin B (E9259-5G) and sudan I (103624-25G) were purchased from Sigma-Aldrich, St.
After 24 h, the medium was changed to phenol red-free RPMI 1640 supplemented with 5% CS-FBS and 2% AbAm and either control (vehicle only), E2, tartrazine, erythrosin B, or sudan I (0.
We evaluated tartrazine, erythrosin B, and sudan I with cell proliferation and reporter gene assays.
001 nM-10 nM, similar to the physiological concentration range of E2 and within estimated human exposure following consumption of the ADI of tartrazine and erythrosin B or wildlife exposure following drinking of tartrazine containing water.