equilibrium dialysis


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e·qui·lib·ri·um di·al·y·sis

in immunology, a method for determination of association constants for hapten-antibody reactions in a system in which the hapten (dialyzable) and antibody (nondialyzable) solutions are separated by semipermeable membranes. Because, at equilibrium the quantity of free hapten will be the same in the two compartments, quantitative determinations can be made of hapten-bound antibody, free antibody, and free hapten.

e·qui·lib·ri·um di·a·ly·sis

(ē'kwi-lib'rē-ŭm dī-al'i-sis)
immunology A method for determination of association constants for hapten-antibody reactions in a system in which the hapten (dialyzable) and antibody (nondialyzable) solutions are separated by semipermeable membranes.
References in periodicals archive ?
GC-MS: Gas chromatography mass spectrometry ILE: Intravenous lipid emulsion LA: Local anaesthetic Log [P.sub.(octanol)]: Logarithmic expression of lipophilic partition coefficient of drugs in octanol and water PBS: Phosphate buffered saline RED: Rapid equilibrium dialysis VIFM: Victorian Institute of Forensic Medicine.
Qian, "A practical method for measuring free drug concentration in whole blood using an equilibrium dialysis method," Thermo Scientific Pierce Research Productions, 2007.
[4] Nonstandard abbreviations: [T.sub.4], thyroxine; TSH, thyroid-stimulating hormone; ALB, albumin; TBG, thyroxine-binding globulin; [TT.sub.4], total thyroxine; [FT.sub.4], free thyroxine; ED, equilibrium dialysis; LC-MS/MS, liquid chromatography-tandem mass spectrometry; IA, immunoassay; NIH-CC, NIH Clinical Center; [T.sub.4]-[d.sub.5], deuterium-labeled L-thyroxine; MRM, multiple reaction monitoring; AUC, area under the curve.
[4] Nonstandard abbreviations: [T.sub.4], 3,3',5,5'-tetraiodo-L-thyronine, thyroxine; [T.sub.3], 3,3',5-triiodo-L-thyronine; rT3, 3',5',3-triiodothyronine or reverse [T.sub.3]; [FT.sub.4], free [T.sub.4]; [FT.sub.3], free [T.sub.3]; IA, immunoassay; ED, equilibrium dialysis; UF, ultrafiltration; ID-MS, isotope dilution mass spectrometry; GC, gas chromatography; SIM, selected ion monitoring; LC, liquid chromatography; MS/MS, tandem mass spectrometry; MRM, multiple reaction monitoring; IS, internal standard; SPE, solid-phase extraction; LLOQ, lower limit of quantification; ULOQ, upper limit of quantification; TSH, thyroid-stimulating hormone.
This was accomplished by progressively diluting serum retentate with serum dialysate (obtained using the equilibrium dialysis method described above).
A theoretically sound and practicable equilibrium dialysis method for measuring percentage of free testosterone.
* Fractions of serum [T.sub.4] obtained by equilibrium dialysis and ultrafiltration.
The dialyzable [T.sub.4] in these solutions (direct equilibrium dialysis) was 7.7 to 125 pmol/L (0.6 to 9.7 ng/dL).
Ultrafltration devices tested for use in a free thyroxine assay validated by comparison with equilibrium dialysis. Scand J Clin Lab Invest 1990;50:663-9.
Confirmation of a normal serum free [T.sub.4] by equilibrium dialysis followed by evidence of increased [T.sub.4] binding by the TTR plasma protein fraction established the diagnosis in this case.
Traditional measurement procedures separate FTe from the protein-bound Te fraction by equilibrium dialysis (ED) or ultrafiltration (UF).
Established methods that comply fairly well with the above-mentioned requirements are based on equilibrium dialysis (1, 5-7) and ultrafiltration (8-10) followed by quantification of the free hormone by RIA in the dialysate/ ultrafiltrate.
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