GC-MS: Gas chromatography mass spectrometry ILE: Intravenous lipid emulsion LA: Local anaesthetic Log [P.sub.(octanol)]: Logarithmic expression of lipophilic partition coefficient of drugs in octanol and water PBS: Phosphate buffered saline RED: Rapid equilibrium dialysis VIFM: Victorian Institute of Forensic Medicine.
Qian, "A practical method for measuring free drug concentration in whole blood using an equilibrium dialysis method," Thermo Scientific Pierce Research Productions, 2007.
[4] Nonstandard abbreviations: [T.sub.4], thyroxine; TSH, thyroid-stimulating hormone; ALB, albumin; TBG, thyroxine-binding globulin; [TT.sub.4], total thyroxine; [FT.sub.4], free thyroxine; ED,
equilibrium dialysis; LC-MS/MS, liquid chromatography-tandem mass spectrometry; IA, immunoassay; NIH-CC, NIH Clinical Center; [T.sub.4]-[d.sub.5], deuterium-labeled L-thyroxine; MRM, multiple reaction monitoring; AUC, area under the curve.
[4] Nonstandard abbreviations: [T.sub.4], 3,3',5,5'-tetraiodo-L-thyronine, thyroxine; [T.sub.3], 3,3',5-triiodo-L-thyronine; rT3, 3',5',3-triiodothyronine or reverse [T.sub.3]; [FT.sub.4], free [T.sub.4]; [FT.sub.3], free [T.sub.3]; IA, immunoassay; ED,
equilibrium dialysis; UF, ultrafiltration; ID-MS, isotope dilution mass spectrometry; GC, gas chromatography; SIM, selected ion monitoring; LC, liquid chromatography; MS/MS, tandem mass spectrometry; MRM, multiple reaction monitoring; IS, internal standard; SPE, solid-phase extraction; LLOQ, lower limit of quantification; ULOQ, upper limit of quantification; TSH, thyroid-stimulating hormone.
This was accomplished by progressively diluting serum retentate with serum dialysate (obtained using the equilibrium dialysis method described above).
A theoretically sound and practicable equilibrium dialysis method for measuring percentage of free testosterone.
* Fractions of serum [T.sub.4] obtained by equilibrium dialysis and ultrafiltration.
The dialyzable [T.sub.4] in these solutions (direct equilibrium dialysis) was 7.7 to 125 pmol/L (0.6 to 9.7 ng/dL).
Ultrafltration devices tested for use in a free thyroxine assay validated by comparison with
equilibrium dialysis. Scand J Clin Lab Invest 1990;50:663-9.
Confirmation of a normal serum free [T.sub.4] by
equilibrium dialysis followed by evidence of increased [T.sub.4] binding by the TTR plasma protein fraction established the diagnosis in this case.
Traditional measurement procedures separate FTe from the protein-bound Te fraction by
equilibrium dialysis (ED) or ultrafiltration (UF).
Established methods that comply fairly well with the above-mentioned requirements are based on
equilibrium dialysis (1, 5-7) and ultrafiltration (8-10) followed by quantification of the free hormone by RIA in the dialysate/ ultrafiltrate.
