This column will focus on describing three methods for assessment of eye irritation: the Bovine Cornea Opacity and Permeability Test (BCOP), the Chorioallantoic Membrane Vascular Assay (CAMVA) and the Epiocular model assay, each has specific advantages and limitations, some will be described here.
The EpiOcular Eye Irritation Test (EIT) utilizes human cells.
Current in vitro products include EpiAirway, EpiDerm, EpiDermFT, EpiGingival, EpiOcular
, EpiOral, EpiVaginal, and MelanoDerm tissue models, and Human Dendritic Cells.
Silvia Letasiova, MatTek Bratislava, presented an eye irritation test (EIT), which utilizes a three-dimensional reconstructed human cornea-like epithelial (RhCE) tissue model, EpiOcular
, based on normal human cells.
Other alternatives include the hen's egg test on the chorioallantoic membrane (HET-CAM assay), reconstructed human corneal epithelium models (EpiOcular
Reproducibility and correlation of EpiOcular
, a three-dimensional tissue culture model of the human corneal epithelium.
--Precision Digital Dispensing of Patterned Picoliter Quantities of Test Material onto Apical Surfaces of Human 3D-Reconstructed Airway Tissues Correlation of Two In Vitro EpiOcular
Test Methods and Consumer Eye Irritation Data for Cleaning Products