epicotyl

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epicotyl

the axis (or stem) of the shoot in a young germinating seedling, located above the COTYLEDON. See GERMINATION and Fig. 171 .
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In vitro organogenesis of Troyer citrange (Citrus sinensis x Poncirus trifoliata) epicotyls cultivated on medium containing 1.0 mg [dm.sup.-3] BAP and 0.1 mg [dm.sup.-3] NAA revealed an average of 10.4 buds per explant, 7.2 of which developed into shoots (Moreira-Dias et al., 2000), values higher than those obtained in our work.
Although many different types of explants such as epicotyls, leaf petiole and internodes have also been used in tomato (Plastira and Perdikaris, 1997; Gubis et al., 2003) but hypocotyls and cotyledons were by far the most commonly used explants in tomato (Jatoi et al., 1995; Soniya et al., 2001; Park et al., 2003; Raj et al., 2005; Roy et al, 2006).
Earlier reports have tried using different explants such as cotyledonary node [15,16], whole cotyledonary node [17], epicotyl and primary leaves [18, 19], primary leaf nodes [20], and hypocotyls [14, 21-24].
Double coty-node explants were obtained by excising out the epicotyls at the cotyledonary junctions and cutting off the hypocotyls 45 mm beneath the cotyledons.
Fresh samples of 0.5 g were taken from the epicotyls and extracted in 10 mL acidified methanol (methanol-water-hydrochloric acid, 79:20:1, v/v) for UV-B absorbing compounds, according to the procedure of Mirecki and Teramura [24].
Nevertheless, the authors indicated that induction of somatic embryos was possible starting from hypocotyls, cotyledons, leaves and epicotyls, observing the formation of globular stages without progression to more advanced stages.
The magnitude of the estimates in the determination of the genotypic coefficient (H2) for both characters that were analyzed were above 82% and values above 98% were observed for the length of the hypocotyl in experiment 2 and for the lengths of the epicotyls in experiments 1 and 2 (Table 1).
Also, it has enabled (Kaewpoo and Te-chato, 2009) to study polyploidization, they confirmed that there is no variation in ploidy level of micropropagated plants derived from both epicotyls and hypocotyls culture.
Effect of carbon source on the shoot proliferation potential of epicotyls explants of Syzygium cuminii.