M2 PHARMA-February 28, 2019-DNA Script Claims World's First Enzymatic Synthesis
of a High-Purity 200-Nucleotide Strand of DNA
Recently, Marie's group demonstrated the authentically successful O-glycosylation of trans-resveratrol to overcome its low water solubility and stability by enzymatic synthesis
of resveratrol [alpha]-glycosides from [beta]-cyclodextrin-resveratrol complex in water .
has several advantages such as mild reaction conditions, low impurities, low energy needs, minimal waste, product easyly isolated and reusable biocatalyst (5,6).
The concept of using in vitro enzymatic synthesis
to amplify DNA was first mooted in 1971 (1) and demonstrated in 1985 as the "polymerase chain reaction" (2).
This paper unveils the synergism effect achieved using the microwave irradiation technique on enzymatic synthesis
under various optimal conditions.
The reduction on enzymatic activity to amylase after 96 hours of SSF can be attributed to a possible nutrient depletion or accumulation of products that inhibit enzymatic synthesis
or microbial growth (Shafique et al., 2009).
(1993) A facile enzymatic synthesis
of cellooligosaccharide derivatives using [beta]-lactosyl fluoride.
Lathi, "Intensification of enzymatic synthesis
of propylene glycol monolaurate from 1,2-propanediol and lauric acid under microwave irradiation: kinetics of forward and reverse reactions," Enzyme and Microbial Technology, vol.
Optimization of enzymatic synthesis
of eugenol ester using statistical approaches.
Although enzymatic synthesis
of bio-based polymers such as PLA was considered, the long reaction time of the enzymatic synthesis
(12 hours) makes it impractical for completion during the laboratory period.
Gene expression begins with the enzymatic synthesis
of an RNA molecule that is a copy of one strand of the DNA segment corresponding to the gene.