37) T20 is a member of a successful new generation of entry inhibitors targeting Env gene
Internal PERV control primers were designed to amplify conserved regions between 5' long terminal repeat (LTR) and 3' env gene
Molecular characterization of the env gene
from Brazilian field isolates of Bovine leukemia virus.
The assay utilizes PCR-amplified env gene
and hybridizes those genes to V3-coding sequences from viruses with known tropisms.
RT-PCR of the env gene
was performed under the following conditions: 40 microliters ([micro]L) of master mix with 0.
The full-length env gene
was selected to stimulate E protein secretion in the form of VLPs in order to expose conformational antigens that may be critical in eliciting protective immune responses.
They examined the most variable region of the virus' env gene
, which encodes a protein forming the outer coat of the virus.
Numerous laboratories have reported that a gene segment that corresponds to a unique 660 base pair sequence of the MMTV envelope gene, known as the env gene
, is present in 30%-40% of fresh and archival breast cancer specimens from patients in the United States, Italy, Mexico, Brazil, and Argentina; 80% in Tunisia; and 10%-12% in China and Japan.
The viral vaccines of the invention are optimally combined with a recombinant HIV env booster, or a recombinant HIV env gene
DNA priming or boosting vaccine.
Phylogenetic analysis of the env gene
of HIV-1 isolates taking into account individual nucleotide substitution rates.
A 345 nucleotide segment of the C2V3C3 region of the env gene
and approximately 400 nucleotides of the p17 coding region of gag were sequenced and analyzed in a blinded fashion.