It is predominantly composed of laminin with other components including heparin sulfate proteoglycan, entactin
, and collagen type IV.
The manufacturer relinquished a vague content for the ECM used in this study which contains entactin
, collagen IV, laminin, and heparin sulfate proteoglycan in different concentrations.
Devastation of type IV collagen, laminin, and entactin
leads to demotion of anchoring fibers and appearance of bullae .
Broekelmann et al., "Degradation of entactin
by matrix metalloproteinases.
Ramadori, "Transforming growth factor-[beta]1 stimulates the synthesis of basement membrane proteins laminin, collagen type IV and entactin
in rat liver sinusoidal endothelial cells," Journal of Hepatology, vol.
Matrigel is a suitable matrix that includes laminins, collagen IV, entactin
, heparan sulfate proteoglycan, as well as various growth factors, which can highly representative of the tumor microenvironment.[sup] With the purpose of long-term proliferation of the organoids, multifold factors were added to the overlaid medium.[sup] Wnt signals are essential for the proliferation of intestinal epithelium, and the Wnt agonist R-spondin 1 induces crypt hyperplasia in vivo .[sup] Signaling by epidermal growth factor (EGF) is associated with intestinal proliferation.[sup] Transgenic expression of Noggin induces an expansion of crypt numbers.[sup] We added R-spondin-1, EGF, and Noggin to Matrigel to sustain ever-increasing CRC organoids.
The STBM comprises extracellular matrix (ECM) proteins, such as laminin, collagen IV, perlecan, and entactin
(Yazama et al., 1997; Siu and Cheng, 2004).
The ECM gel was prepared from Engelbreth-Holm-Swarm (EHS) mouse sarcoma and was composed of laminin as the major component, collagen type IV, heparan sulfate proteoglycan, entactin
and other minor components.
Chung, "The basement membrane glycoprotein entactin
promotes cell attachment and binds calcium ions," The Journal of Biological Chemistry, vol.
These enzymes are able to split denatured collagens, fibronectin, lamin, entactin
, elastin, etc.
Conditional deletion of Ap-2[alpha] in lens placode derivatives, including the corneal epithelium, results in a decrease in the expression of the cell-cell adhesion molecule E-cadherin, misexpression of laminin, entactin
and type IV collagen, and disruption of stromal collagen fibril organization, showing that AP-2[alpha] is required for proper formation of the mouse cornea [179,182].
(5) MMP-7 displays strong proteolytic activity against a wide range of ECM components including collagen types IV, V, IX, and X, proteoglycans, fibronectin, laminin, gelatin, elastin, and entactin
. It also cleaves various non-ECM bioactive molecules such as Fas ligand, pro-tumor necrosis factor [alpha], E-cadherin, [beta]4-integrin, insulin-like growth factor binding protein 3 and insulin-like growth factor binding protein 5, and [alpha]-defensin.