Overall, results show that endospores
of lactate-fermenting, gas-producing Clostridium spp.
botulinum can also cause infant botulism (when infants ingest the endospores
and the toxin forms in the intestines in the absence of mature gastrointestinal normal flora) and wound botulism (where the bacterium multiplies in a wound and produces toxin which is absorbed into the bloodstream).
fused to a thin, flat exospore, and an endospore
adjacent to the
produced by these isolates become visible inside the vegetative cell after 18 h of incubation (Fig.
Definitive diagnosis of coccidioidomycosis is made when the organism is recovered from respiratory secretions, body fluids or tissue especially detecting the characteristic spherules with endospores
. (9) Fungal hyphae can be recovered from specimens obtained from boundaries of old cavitary lung lesions and skin lesions.
In case of endospore
formation test isolate 1, 2, 7, 12, 13, 14, 18, 19, 23, 24, 29, 33, 34, 40, 44, 45, 46, 51, 52, 56, 57, 61, 62, 68 and 69 indicated positive test, therefore confirming the rhizobacteria being Bacillus spp., as by adopting differential staining technique the endospores
retained the primary dye i.e., malachite green, whereas, the vegetative cells lost the stain.
anthracis spores; therefore, the study focused on the use of brain heart infusion (BHI) (Becton Dickinson, Sparks, Maryland) and tryptic soy broth (TSB) (Becton Dickinson, Sparks, Maryland) as nutrients for the germination of Bt spores in the endospore
The morphological characteristics were identified by culturing the isolate on LB agar plates and studying the shape, size, color, opacity, texture, elevation, spreading nature and margin of the colonies, followed by gram's staining, aerobic test, motility test and endospore
Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore
In these cases, the spherule/ endospore
form has been implicated in human disease after exposure to infected tissues or fluids.
Effect of sonic stimulation on Bacillus endospore
Biochemical characterization and phylogenetic relatedness of strains isolated from Thar Desert Characteristics 1S 2AS 2BS Gram staining + + + Cell size 0.1-0.3x 0.2-0.3x 0.5-0.8x (width, length [micro]m) 1-2 1-3 1-4 Motility + + + Endospore
+ + + Temperature 25-55 30-55 30-60 range ([degrees]C) pH range 6-8 6-8 6-8 Indole - - - Triple sugar Iron + + - MR - - - VP + + - Nitrate + + + H2S - - - Citrate + + - Catalase + + + Oxidase + + + Phylogenetic Bacillus Bacillus Aeribacillus similarity subtilis subtilis pallidus subsp.