endonucleases


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endonucleases

Enzymes that can split DNA or RNA at any point along the molecule by cutting PHOSPHODIESTER BONDS.
References in periodicals archive ?
A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences.
For the validation of TALEN-mediated homologous recombination via a T7 endonuclease assay, porcine fibroblasts were transfected with the TALEN expression vector by electroporation and the genomic DNA of the transfected fibroblasts was isolated using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma-Aldrich, St.
However, the presence of these mutations, including substitutions and missense mutations, did not affect the restriction-enzyme cutting sites of other endonucleases (Hinc II, Acc I, and PflM I; Fig.
To resolve these cases, the amplified product was also digested with AflIII restriction endonuclease.
In addition to homing endonucleases, scientists have been tinkering with two artificial protein systems as programmable gene-editing tools.
For confirmation of cloning, colony-PCR, restriction endonucleases digestion and sequencing methods were employed.
A figura 1B mostra os amplicons S2 de tres cepas isoladas de amostras de salsichas tipo hot dog a granel, submetidos individualmente a reacao enzimatica com a endonuclease XmnI, o que resultou em fragmentos de 770pb e 120pb, caracteristicos das especies L.
10) The H-N-H motif is the 30-33 amino acids consensus sequence containing two pairs of conserved histidines surrounding a conserved asparagine and found in various nucleases including E-group DNase colicins such as colicin E7 (32), and E9 (33) and homing endonucleases (34).
Hamilton Robotics, a company that offers a wide range of liquid handling workstations and laboratory automation solutions, has collaborated with New England Biolabs, a company that offers restriction enzymes, endonucleases, recombinant enzymes, PCR qPCR reagents, expression systems, markers, competent cells and reagents.
This cloning method allows transferring of DNA fragment into different cloning vectors without using restriction endonucleases and ligase.
Two (mu) L of each amplicon were digested with EcoRI, AluI, HhaI and TaqI endonucleases, according to the manufacturers" instruction (Promega, USA) at 37degC (65degC for TaqI) overnight.