endonucleases


Also found in: Dictionary, Thesaurus, Encyclopedia.

endonucleases

Enzymes that can split DNA or RNA at any point along the molecule by cutting PHOSPHODIESTER BONDS.
References in periodicals archive ?
Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences, USA 76:5269-5273.
The focus of the collaboration is to discover novel programmable DNA endonucleases or nickases with high fidelity and catalytic activity as well as novel transduction approaches.
To construct the physical map of the chromosome of Psm, three rare-cutting restriction endonucleases were chosen based on G+C content of P syringae strains, several enzymes with A+T rich recognition sequences were tested, among these enzymes PacI (TTAATTAA), PmeI (GTTTAAAC) and SwaI (ATTTAAAT) were selected which originated 14, 15 and 16 fragments, respectively.
Generally, to produce sticky or complementary ends for sticky-end DNA cloning, insert DNA and vector are separately cut with same restriction endonuclease enzymes.
A variation of fragments from 90 to 500bp was obtained after digestion with the restriction endonucleases Rsa I, Alu I, HinfI, Hpa II, Hha I, Xho I, Acc I, Eco RI and Taq I (Tables 1 and 2).
Four restriction endonucleases were used to differentiate the shrimp species: DdeI, MboI, MboII, and MseI.
If the same approach were to be applied to the detection of the placenta-derived hypomethylated SERPINB5 molecules, an endonuclease that exhibits an opposite action to the methylation-sensitive restriction endonucleases described above would be needed.
coli O157:H7 isolates with two or more restriction endonuclease digestions or using other subtyping methods, such as phage typing, to determine whether such isolates are truly related, even if these isolates have produced matching patterns using a single enzyme digestion (7-9).
The imbalance of calcium homeostasis finally results in calcium-dependent endonuclease activation, which is believed to initiate chromatin fragmentation, another key feature of apoptotic cells (35,36).
For each restriction endonuclease, each fragment profile was compared against those in related species (J.
Nucleases catalyzing DNA and RNA cleavage are indispensable for life, nuclease activities are integral parts of DNA replication; the 5' to 3' exo-and endonucleases are needed to remove RNA primers, and the 3' to 5' exonuclease for proofreading (6).
Vertex will pay USD175m upfront for the exclusive worldwide rights to CRISPR Therapeutics' present and future intellectual property, including foundational CRISPR/Cas9 technology, novel endonucleases, single and double cut guide RNAs, and AAV vectors for DMD and DM1 gene editing products.