Individual human samples were inoculated in 9-day-old embryonated chicken eggs
and amnio-allantoic fluid (AAF) harvested after 48 h incubation.
By using viral inoculation (VI) technique sample from each bird was inoculated in 9-days old pathogen free embryonated chicken eggs
Embryonated chicken eggs
of 9 day-old were inoculated with field isolate virus at the rate 0.2ml/egg following Ali et al.
This finding agrees with findings by other researchers that IBDV strains do not grow easily in embryonated chicken eggs
and take time to adapt .
Each sample was titrated on embryonated chicken eggs
to determine the virus yield .
Collection of embryonated chicken eggs
and Swiss albino mice
Because the patient had collected eggs in a farm where HPAI A (H7N3) virus was detected, the Institute for Epidemiological Diagnosis and Reference, Mexico, tested ocular swabs from both of her eyes for influenza A (H7) by real-time reverse transcription-polymerase chain reaction (rRT-PCR), and embryonated chicken eggs
were inoculated for viral isolation.
The present study also revealed an eclipse phase in vertebrate cell lines and embryonated chicken eggs
during which period, no virus replication could be detected.
"However, classical manufacturing methods for vaccines fail to satisfy this demand," Krammer added.Traditional influenza vaccines, which are produced in embryonated chicken eggs
, can be manufactured in the quantities needed for seasonal strains of influenza.
Besides, the cell-based production process is not dependent on the availability of embryonated chicken eggs
, while offering an alternative to people allergic to chicken proteins.
As done in vaccine production, the seasonal influenza A (H1Nl) viruses used in the study were propagated in embryonated chicken eggs
, and the study's novel influenza A (HlNl) virus was grown in Mandin-Darby canine kidney cells.
Concurrently, to assess retention of vaccinia virus infectivity in RNAlater-preserved organ tissue, embryonated chicken eggs
were infected with 100 plaque forming units (PFU) of vaccinia virus and dissected after 3 days of incubation.