The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs
through chorioallantoic membrane.
Embryonated chicken eggs
of 9 day-old were inoculated with field isolate virus at the rate 0.
One virus (DK/TH/CU-1) was inoculated into embryonated chicken eggs
This finding agrees with findings by other researchers that IBDV strains do not grow easily in embryonated chicken eggs
and take time to adapt .
Collection of embryonated chicken eggs
and Swiss albino mice
The present study also revealed an eclipse phase in vertebrate cell lines and embryonated chicken eggs
during which period, no virus replication could be detected.
Traditional influenza vaccines, which are produced in embryonated chicken eggs
, can be manufactured in the quantities needed for seasonal strains of influenza.
Besides, the cell-based production process is not dependent on the availability of embryonated chicken eggs
, while offering an alternative to people allergic to chicken proteins.
As done in vaccine production, the seasonal influenza A (H1Nl) viruses used in the study were propagated in embryonated chicken eggs
, and the study's novel influenza A (HlNl) virus was grown in Mandin-Darby canine kidney cells.
Concurrently, to assess retention of vaccinia virus infectivity in RNAlater-preserved organ tissue, embryonated chicken eggs
were infected with 100 plaque forming units (PFU) of vaccinia virus and dissected after 3 days of incubation.
The vaccine is prepared from influenza vaccine viruses that are propagated in embryonated chicken eggs
Isolation was carried out on embryonated chicken eggs