Production of embryoids
and calli from isolated microspores of tomato (Lycopersicon esculentum Mill.
To study spontaneous differentiation 4-day-old embryoid
bodies were plated in tissue culture dishes in standard embryonic stem cell media without LIF while for the neuronal differentiation study embryonic stem cells were subjected to an 8-day induction procedure that consisted of 4 days of culture as aggregates without RA followed by 4 days of culture in the presence of 1 [micro]M RA ('4-/4+').
Among the different kinds of cells within the embryoid
bodies were germ cells destined to become sperm.
ES-like cells forming typical embryoid
bodies (EBs) after 5 days were transferred to confocal dishes coated with 0.
Dupuis, et al,  studied the effect of plant donor tissue and isolation procedure effect on early embryoid
formation from protoplasts of sunflower.
melanogaster and Zebrafish), to delicate large cells or cell clusters (adipocytes, embryoid
bodies, pancreatic islets, duct cells and hepatocytes), to seeds (Arabidopsis) and beads (cells growing in or on beads, combi-chem libraries and bead-based assays).
Inhibition of tumor-induced angiogenesis and matrix-metalloproteinase expression in confrontation cultures of embryoid
bodies and tumor spheroids by plant ingredients used in traditional Chinese medicine.
In further studies, the iPS cells readily developed into clusters of cells called embryoid
bodies from which cells of virtually any type can develop.
This is precisely the radical difference between an individual in development and a simple cell growth more or less of an embryoid
They were allowed to mature for two or three weeks inside the embryoid
bodies before being located and removed.
Several media including MS and MN 6 (Murashige and Skoog, 1962; Chu and Hill, 1988) seem to work successfully for embryoid
development, and the composition of most media contains adequate nutrients to feed developing embryoids
As they divided, the stem cells aggregated into microscopic clumps called embryoid