Explants directly originating somatic embryos have
embryogenic route-predetermined cells, because their constitution presents meristematic parental cells.
Histological analysis: the morphological features of the
embryogenic calli and somatic embryos, histological analyses were determined according to the literature (Boissot, Valdez, & Guiderdoni, 1990; Maataoui, Espagnac, & Michaux-Ferriere, 1990; Sandoval, 2005).
The expression of the CnCDKA gene was higher in the
embryogenic callus formation phase, coinciding with its in situ location with meristematic cell centers from which somatic embryos developed.
The
embryogenic calli were placed in regeneration media and incubated in the dark at 26 [+ or -] 2[degrees]C for 4-5 days and under fluorescent
Although the acetic carmin test was not performed for the verification of viable embryos in these callus structures, it was observed that the results obtained were given by the explants, which are proven capable of originating an indirect response, either organogenic or
embryogenic, where initially there was a differentiation (callus) and the arising of friable regions capable of causing sprouts in most treatments.
Somatic embryogenesis and plant regeneration in avocado (Persea Americana Mill.): influence of
embryogenic culture type.
Difference between nonembryogenic and
embryogenic callus was achieved on the basis of callus physical characteristic [21, 22].
Tissue samples were taken for the anatomical study at each developmental stage from the leaf sections and cotyledon segments, including the
embryogenic and organogenic callus.
Pfister, "Stone containing pyelocaliceal diverticulum:
embryogenic, anatomic, radiologic and clinical characteristics," The Journal of Urology, vol.
In parallel, at the beginning of the experiment, three 2-L beaker replicates derived from the incubation tank were prepared with 20 oocytes/mL to estimate the timing of the different
embryogenic stages.
The aim of this study was to determine the cytotoxic and apoptotic/necrotic activity of various types of A vera leaf extracts and AE in a type of skin cancer, B16F10 murine melanoma relative to normal fibroblast cell line (NIH3T3 mouse
embryogenic fibroblast cells) and to investigate the underlying mechanisms.
(2008) reported that the majority of Dendrobium candidum PLBs produced secondary PLBs originated from cells of the interior or exterior of the
embryogenic callus.
