Plants have the ability to propagate though asexual means, such as vegetative apomixis or via in planta somatic embryogenesis
, as long as the parent plant is still alive [15, 16].
This is a highly unusual finding and probably reflects abnormal embryogenesis
or divergent differentiation.2
is a tool of high potential in the improvement of productivity since it allows, among other applications, the propagation of selected genotypes at a large scale, the production of artificial seeds and the cryopreservation of material, as well as providing a method to regenerate genetically modified plants (Vicient and Martinez, 1998; Von Arnold, 2008).
is a biological process that allows the production of an unlimited number of embryos from a cell or group of somatic cells .
from various parts of Muscari comosum var.
The somatic embryogenesis
induction appears as the possibility of using other plant regions or organs, as well as of producing large numbers of embryos from haploid or somatic cells in order to develop a complete plant, with higher genetic integrity when compared with plants regenerated through the process of organogenesis (GRANDO et al., 2013).
One of the processes of plant micropropagation through somatic embryogenesis
involves the in vitro formation of bipolar structures (somatic embryos) from somatic cells under specific culture conditions.
(SE) is used to propagate plants commercially or to study basic aspects of induction and development of somatic embryos.
With the aim of establishing sterilized explants in vitro, in order to callus production and evaluation of embryogenesis
, 32 Hormonal Treatments were used in two separate tests with different concentrations and types.
is a regenerative process of plants in which bipolar structures, similar to zygotic embryos, are formed by somatic cells with no vascular connection to the tissues of the initial explant (Zimmerman, 1993; Jiang et al., 2012), making an asexual propagation system, especially for woody and palmaceous species considered difficult to regenerate by conventional asexual propagation methods (Kielse et al., 2007; Moura et al., 2008).